Artículo
Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites
Pérez Brandan, Cecilia María
; Mesias, Andrea Cecilia
; Acuña, Leonardo
; Teixeira, Thaise Lara; Silva, Claudio Vieira da
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Fecha de publicación:
04/2019
Editorial:
Fundação Oswaldo Cruz
Revista:
Memórias do Instituto Oswaldo Cruz
ISSN:
0074-0276
e-ISSN:
1678-8060
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerization in the host cell. Our goal was to evaluate if the additional supplementation of rP21 during a prime/boost/challenge scheme with T.cruzi TCC attenuated parasites could modify the well known protective behavior conferred by these parasites. The humoral immune response was evaluated through the assessment of total anti-T. cruzi antibodies as well as IgG subtypes. IFN-, TNF- and IL-10 were measured in supernatants of splenic cells stimulated with total parasite homogenate or rP21. Our results demonstrated that, when comparing TCC+ rP21 vs. TCC vaccinated animals, the levels of IFN- were significantly higher in the former group, while the levels of IL-10 and TNF- were significantly lower. Further, the measurement of parasite load after lethal challenge showed an exacerbated infection and parasite load in heart and skeletal muscle after pretreatmentwith rP21, suggesting the important role of this protein during parasite natural invasion process. In conclusion, our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites.
Palabras clave:
ATTENUATED VACCINE
,
CHAGAS DISEASE
,
P21
,
TRYPANOSOMA CRUZI
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Articulos(IPE)
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Citación
Pérez Brandan, Cecilia María; Mesias, Andrea Cecilia; Acuña, Leonardo; Teixeira, Thaise Lara; Silva, Claudio Vieira da; Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by trypanosoma cruzi attenuated parasites; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 4; 4-2019; 1-7
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