Artículo
The exocrine pancreas is an extracardiac source of atrial natriuretic peptide
Najenson, Ana Clara
; Bianchi, Mariana
; Courreges, Ana Paula
; Rodriguez, Myrian Roxana
; Casco, Víctor H.; Vatta, Marcelo Sergio
; Bianciotti, Liliana Graciela
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Fecha de publicación:
06/2019
Editorial:
Springer
Revista:
Pflugers Archiv-European Journal of Physiology
ISSN:
0031-6768
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Previous studies have shown that atrial natriuretic peptide (ANP) regulates exocrine pancreatic function in health and disease. As extracardiac sources of ANP have been identified and ANP-like immunoreactivity has been reported in the exocrine pancreas, in the present work we sought to establish whether ANP was produced in the rat exocrine pancreas and if conditions like fasting/feeding or acute pancreatitis were reflected on ANP expression. By using RT-PCR, immunoblotting, and immunofluorescence microscopy assays, it was found that both mRNA and protein ANP were present in the acinar cells of the exocrine pancreas. The amount of ANP in the pancreas was lower in than the atrium but similar to other tissues like the kidney and liver. Immunogold labeling electron microscopy studies revealed that ANP was localized in zymogen granules and the endoplasmic reticulum suggesting local synthesis and package into granules. ANP protein expression was significantly increased not only in fasting but also in acute pancreatitis, the latter probably related to impaired secretion. Natriuretic peptide receptor type C which mediates ANP biological effects in the exocrine pancreas was also present in acinar cells and its expression did not change with either fasting or acute pancreatitis. Present findings show that the exocrine pancreas is a relatively important extracardiac source of ANP and further support previous studies strongly suggesting the active role of the peptide in pancreatic physiology and pathophysiology.
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Identificadores
Colecciones
Articulos(INIGEM)
Articulos de INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Articulos de INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Articulos(IQUIMEFA)
Articulos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Articulos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Citación
Najenson, Ana Clara; Bianchi, Mariana; Courreges, Ana Paula; Rodriguez, Myrian Roxana; Casco, Víctor H.; et al.; The exocrine pancreas is an extracardiac source of atrial natriuretic peptide; Springer; Pflugers Archiv-European Journal of Physiology; 471; 6; 6-2019; 915-924
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