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Artículo

Inhibitory role of ERβ on anterior pituitary cell proliferation by controlling the expression of proteins related to cell cycle progression

Pérez, Pablo AníbalIcon ; Petiti, Juan PabloIcon ; Wagner, Ignacio A.; Sabatino, María EugeniaIcon ; Sasso, Corina VerónicaIcon ; de Paul, Ana LuciaIcon ; Torres, Alicia InesIcon ; Gutiérrez, SilvinaIcon
Fecha de publicación: 11/2015
Editorial: Elsevier Ireland
Revista: Molecular and Cellular Endocrinology
ISSN: 0303-7207
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Considering that the role of ERβ in the growth of pituitary cells is not well known, the aim of this work was to determine the expression of ERβ in normal and tumoral cells and to investigate its implications in the proliferative control of this endocrine gland, by analyzing the participation of cyclin D1, Cdk4 and p21. Our results showed that the expression of ERβ decreased during pituitary tumoral development induced by chronic E2 stimulation. The 20 ± 1.6% of normal adenohypophyseal cells expressed ERβ, with this protein being reduced in the hyperplastic/adenomatous pituitary: at 20 days the ERβ+ population was 10.7 ± 2.2%, while after 40 and 60 days of treatment an almost complete loss in the ERβ expression was observed (40d: 1 ± 0.6%; 60d: 2 ± 0.6%). The ERα/β ratio increased starting from tumors at 40 days, mainly due to the loss of ERβ expression. The cell proliferation was analyzed in normal and hyperplastic pituitary and also in GH3β- and GH3β+ which contained different levels of ERβ expression, and therefore different ERα/β ratios. The over-expression of ERβ inhibited the GH3 cell proliferation and expression of cyclin D1 and ERα. Also, the ERβ activation by its agonist DPN changed the subcellular localization of p21, inducing an increase in the p21 nuclear expression, where it acts as a tumoral suppressor. These results show that ERβ exerts an inhibitory role on pituitary cell proliferation, and that this effect may be partially due to the modulation of some key regulators of the cell cycle, such as cyclin D1 and p21. These data contribute significantly to the understanding of the ER effects in the proliferative control of pituitary gland, specifically related to the ERβ function in the E2 actions on this endocrine gland.
Palabras clave: CELL PROLIFERATION , CYCLIN D1 , ESTRADIOL , ESTROGEN RECEPTOR Β , P21 , PITUITARY
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/12026
DOI: http://dx.doi.org/10.1016/j.mce.2015.08.009
URL: http://www.sciencedirect.com/science/article/pii/S0303720715300496
Colecciones
Articulos(INICSA)
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Citación
Pérez, Pablo Aníbal; Petiti, Juan Pablo; Wagner, Ignacio A.; Sabatino, María Eugenia; Sasso, Corina Verónica; et al.; Inhibitory role of ERβ on anterior pituitary cell proliferation by controlling the expression of proteins related to cell cycle progression; Elsevier Ireland; Molecular and Cellular Endocrinology; 415; 11-2015; 100-113
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