Artículo
Pre-ischemic enriched environment increases retinal resilience to acute ischemic damage in adult rats
González Fleitas, María Florencia
; Aranda, Marcos Luis
; Dieguez, Hernán
; Devouassoux, Julián Daniel; Chianelli, Mónica Silvia
; Dorfman, Damián
; Rosenstein, Ruth Estela
Fecha de publicación:
01/2019
Editorial:
Academic Press Ltd - Elsevier Science Ltd
Revista:
Experimental Eye Research
ISSN:
0014-4835
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Retinal ischemia is a condition associated with several degenerative diseases leading to visual impairment and blindness worldwide. Currently, there is no highly effective therapy for ischemic retinopathies. This study was designed to determine possible benefits of pre-exposure to enriched environment against retinal damage induced by acute ischemia. For this purpose, adult male Wistar rats were randomly assigned to a pre-ischemic standard environment or a pre-ischemic enriched environment for 3 weeks, followed by unilateral ischemia induced by increasing intraocular pressure above 120 mm Hg for 40 min and reperfusion for 1 or 2 weeks in standard environment. Animals were subjected to electroretinography and histological analysis. Pre-ischemic enriched environment afforded significant functional protection in eyes exposed to ischemia/reperfusion injury. A marked reduction in retinal layer thickness, reduced synaptophysin-immunoreactivity and retinal ganglion cell (RGC) number, and increased microglia/macrophage reactivity were observed in ischemic retinas from animals submitted to pre-ischemic standard environment, which were prevented by pre-ischemic enriched environment. A deficit in anterograde transport from the retina to the superior colliculus and the lateral geniculate nucleus was observed in animals exposed to pre-ischemic standard environment, which was lower in animals previously exposed to enriched environment. The exposure to enriched environment before ischemia increased retinal brain derived neurotrophic factor (BDNF) protein levels in ischemic retinas and the administration of ANA-12 (a TrkB antagonist) abolished the protective effect of enriched environment on retinal function and retinal ganglion cell number. These results indicate that pre-ischemic enriched environment increases retinal resilience to acute ischemic damage, possibly through a BDNF/TrkB mediated pathway.
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Colecciones
Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Citación
González Fleitas, María Florencia; Aranda, Marcos Luis; Dieguez, Hernán; Devouassoux, Julián Daniel; Chianelli, Mónica Silvia; et al.; Pre-ischemic enriched environment increases retinal resilience to acute ischemic damage in adult rats; Academic Press Ltd - Elsevier Science Ltd; Experimental Eye Research; 178; 1-2019; 198-211
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