Artículo
The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
Solari, Claudia María
; Petrone Parcero, María Victoria
; Toro, Ayelen Rayen
; Vazquez Echegaray, Camila
; Cosentino, María Soledad
; Waisman, Ariel
; Francia, Marcos Gabriel
; Barañao, Jose Lino Salvador
; Miriuka, Santiago Gabriel
; Guberman, Alejandra Sonia
Fecha de publicación:
07/2019
Editorial:
BioMed Central
Revista:
BMC Research Notes
ISSN:
1756-0500
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Objective: Redox homeostasis maintenance is essential to bring about cellular functions. Particularly, embryonic stem cells (ESCs) have high fidelity mechanisms for DNA repair, high activity of different antioxidant enzymes and low levels of oxidative stress. Although the expression and activity of antioxidant enzymes are reduced throughout the differentiation, the knowledge about the transcriptional regulation of genes involved in defense against oxidative stress is yet restricted. Since glutathione is a central component of a complex system involved in preserving cellular redox status, we aimed to study whether the expression of the glutathione reductase (Gsr) gene, which encodes an essential enzyme for cellular redox homeostasis, is modulated by the transcription factors critical for self-renewal and pluripotency of ESCs. Results: We found that Gsr gene is expressed in ESCs during the pluripotent state and it was upregulated when these cells were induced to differentiate, concomitantly with Nanog decreased expression. Moreover, we found an increase in Gsr mRNA levels when Nanog was downregulated by a specific shRNA targeting this transcription factor in ESCs. Our results suggest that Nanog represses Gsr gene expression in ESCs, evidencing a role of this crucial pluripotency transcription factor in preservation of redox homeostasis in stem cells.
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Solari, Claudia María; Petrone Parcero, María Victoria; Toro, Ayelen Rayen; Vazquez Echegaray, Camila; Cosentino, María Soledad; et al.; The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells; BioMed Central; BMC Research Notes; 12; 1; 7-2019; 1-7
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