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dc.contributor.author
García, I. L.
dc.contributor.author
Lopez Jimenez, Jimmy Andres
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dc.contributor.author
Dorado, M. P.
dc.contributor.author
Kopsahelis, N.
dc.contributor.author
Alexandri, M.
dc.contributor.author
Papanikolaou, S.
dc.contributor.author
Villar, Marcelo Armando
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dc.contributor.author
Koutinas, A. A.
dc.date.available
2017-01-25T20:53:53Z
dc.date.issued
2013-02
dc.identifier.citation
García, I. L.; Lopez Jimenez, Jimmy Andres; Dorado, M. P.; Kopsahelis, N.; Alexandri, M.; et al.; Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator; Elsevier; Bioresource Technology; 130; 2-2013; 16-22
dc.identifier.issn
0960-8524
dc.identifier.uri
http://hdl.handle.net/11336/11963
dc.description.abstract
Utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (PHA) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. Batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) with a 2.8–8% 3HV content. Fed-batch fermentations in shake flasks led to the production of 10.9 g/L P(3HB-co-3HV) and a 55.6% P(3HB-co-3HV) content. NaCl concentrations between 2 and 6 g/L gradually became inhibitory to bacterial growth and PHA formation, whereas in the case of K2SO4, the inhibitory effect was observed only at concentrations higher than 20 g/L. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and nuclear magnetic resonance (13C NMR) demonstrated that the incorporation of 3HV into the obtained P(3HB-co-3HV) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. Integrating PHA production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Crude Glycerol
dc.subject
Rapeseed Meal Hydrolysate
dc.subject
Microbial Bioconversion
dc.subject
Cupriavidus Necator
dc.subject
Polyhydroxyvalkanoates
dc.subject.classification
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
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dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Evaluation of by-products from the biodiesel industry as fermentation feedstocks for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-01-24T18:30:33Z
dc.journal.volume
130
dc.journal.pagination
16-22
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: García, I. L.. Universidad de Córdoba; España
dc.description.fil
Fil: Lopez Jimenez, Jimmy Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
dc.description.fil
Fil: Dorado, M. P.. Universidad de Córdoba; España
dc.description.fil
Fil: Kopsahelis, N.. Agricultural University of Athens; Grecia
dc.description.fil
Fil: Alexandri, M.. Agricultural University of Athens; Grecia
dc.description.fil
Fil: Papanikolaou, S.. Agricultural University of Athens; Grecia
dc.description.fil
Fil: Villar, Marcelo Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
dc.description.fil
Fil: Koutinas, A. A.. Agricultural University of Athens; Grecia
dc.journal.title
Bioresource Technology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S096085241201783X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.biortech.2012.11.088
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