Artículo
Characterization of the Na+/HCO3 - Cotransport in Human Neutrophils
Giambelluca, Miriam Soledad
; Ciancio, Maria Carolina
; Orlowski, Alejandro
; Gende, Oscar Alfredo
; Pouliot, Marc; Aiello, Ernesto Alejandro
Fecha de publicación:
04/2014
Editorial:
Karger
Revista:
Cellular Physiology And Biochemistry
ISSN:
1015-8987
e-ISSN:
1421-9778
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pHi ) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils.
Methods: Freshly isolated human neutrophils acidified by an ammonium prepulse were used in this study.
Results: We demonstrated that resting neutrophils have a bicarbonate transport mechanism that prevents acidification when the Na+/H+ exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na+/HCO3 - cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. In western blot and RTPCR analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was stimulated with fMLF/ cytochalasin B. This increase in the removal of acid equivalents was insensitive to the blockade of the NADPH oxidase with DPI.
Conclusion: It is concluded that neutrophils have an NBC that regulates basal pHi and is modulated by chemotactic agents.
Palabras clave:
Intracellular Ph
,
Ion Transport
,
Blood Cells
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Identificadores
Colecciones
Articulos(CIC)
Articulos de CENTRO DE INVEST.CARDIOVASCULARES (I)
Articulos de CENTRO DE INVEST.CARDIOVASCULARES (I)
Citación
Giambelluca, Miriam Soledad; Ciancio, Maria Carolina; Orlowski, Alejandro; Gende, Oscar Alfredo; Pouliot, Marc; et al.; Characterization of the Na+/HCO3 - Cotransport in Human Neutrophils; Karger; Cellular Physiology And Biochemistry; 33; 4; 4-2014; 982-989
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