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dc.contributor.author Vargas, Lorena Alejandra
dc.contributor.author Diaz, Romina Gisel
dc.contributor.author Swenson, Erik R.
dc.contributor.author Perez, Nestor Gustavo
dc.contributor.author Alvarez, Bernardo
dc.date.available 2017-01-25T20:32:59Z
dc.date.issued 2013-07
dc.identifier.citation Vargas, Lorena Alejandra; Diaz, Romina Gisel; Swenson, Erik R.; Perez, Nestor Gustavo; Alvarez, Bernardo; Inhibition of carbonic anhydrase prevents the Na /H exchanger 1-dependent slow force response to rat myocardial stretch; American Physiological Society; American Journal Of Physiology-heart And Circulatory Physiology; 305; 2; 7-2013; 228-237
dc.identifier.issn 0363-6135
dc.identifier.uri http://hdl.handle.net/11336/11951
dc.description.abstract Myocardial stretch is an established signal that leads to hypertrophy. Myocardial stretch induces a first immediate force increase followed by a slow force response (SFR), which is a consequence of an increased Ca2+ transient that follows the NHE1 Na+/H+ exchanger activation. Carbonic anhydrase II (CAII) binds to the extreme COOH terminus of NHE1 and regulates its transport activity. We aimed to test the role of CAII bound to NHE1 in the SFR. The SFR and changes in intracellular pH (pHi) were evaluated in rat papillary muscle bathed with CO2/HCO3− buffer and stretched from 92% to 98% of the muscle maximal force development length for 10 min in the presence of the CA inhibitor 6-ethoxzolamide (ETZ, 100 μM). SFR control was 120 ± 3% (n = 8) of the rapid initial phase and was fully blocked by ETZ (99 ± 4%, n = 6). The SFR corresponded to a maximal increase in pHi of 0.18 ± 0.02 pH units (n = 4), and pHi changes were blocked by ETZ (0.04 ± 0.04, n = 6), as monitored by epifluorescence. NHE1/CAII physical association was examined in the SFR by coimmunoprecipitation, using muscle lysates. CAII immunoprecipitated with an anti-NHE1 antibody and the CAII immunoprecipitated protein levels increased 58 ± 9% (n = 6) upon stretch of muscles, assessed by immunoblots. The p90RSK kinase inhibitor SL0101–1 (10 μM) blocked the SFR of heart muscles after stretch 102 ± 2% (n = 4) and reduced the binding of CAII to NHE1, suggesting that the stretch-induced phosphorylation of NHE1 increases its binding to CAII. CAII/NHE1 interaction constitutes a component of the SFR to heart muscle stretch, which potentiates NHE1-mediated H+ transport in the myocardium.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Physiological Society
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Stretch
dc.subject Carbonic anhydrase II
dc.subject Slow force response
dc.subject.classification Fisiología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Inhibition of carbonic anhydrase prevents the Na /H exchanger 1-dependent slow force response to rat myocardial stretch
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-01-25T13:57:21Z
dc.identifier.eissn 1522-1539
dc.journal.volume 305
dc.journal.number 2
dc.journal.pagination 228-237
dc.journal.pais Estados Unidos
dc.journal.ciudad Bethesda
dc.description.fil Fil: Vargas, Lorena Alejandra. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
dc.description.fil Fil: Diaz, Romina Gisel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
dc.description.fil Fil: Swenson, Erik R.. University of Washington; Estados Unidos
dc.description.fil Fil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
dc.description.fil Fil: Alvarez, Bernardo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
dc.journal.title American Journal Of Physiology-heart And Circulatory Physiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1152/ajpheart.00055.2013
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://ajpheart.physiology.org/content/305/2/H228


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  • Articulos(CIC) [120]
    Articulos de CENTRO DE INVEST.CARDIOVASCULARES (I)

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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)