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Artículo

Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering

Torres, María LuzIcon ; Fernandez, María JoséIcon ; Dellatorre, Fernando GasparIcon ; Cortizo, Ana María; Oberti, Tamara GiselaIcon
Fecha de publicación: 06/2019
Editorial: Elsevier
Revista: Algal Research
ISSN: 2211-9264
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biomateriales

Resumen

There is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from Undaria pinnatifida, a brown sea weed invasive in Argentinian coast. The isolated alginate was structurally characterized by IR and 1H NMR spectroscopies, intrinsic viscosity and TGA. For comparison purposes, commercial sodium alginate was purified and characterized using the same protocol as for raw material. Toxicity and biocompatibility of sodium alginate obtained from algae were studied using a murine macrophage-like cell line (RAW 264.7) and bone marrow stromal cells (BMSC), respectively. The presence of impurities inhibited both RAW 264.7 and bone marrow stromal cell proliferation and increased nitric oxide production from macrophages, while inhibited osteoblastic differentiation of BMSC. All these effects were reverted by the purification of alginate. In conclusion, alginate purification improves biocompatibility and osteo-induction while decreases its toxicity.
Palabras clave: BIOCOMPATIBILITY , CYTOTOXICITY , PURIFICATION , SODIUM ALGINATE , STRUCTURAL CHARACTERIZATION
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/118924
URL: https://www.sciencedirect.com/science/article/pii/S2211926418308506?via%3Dihub
DOI: https://doi.org/10.1016/j.algal.2019.101499
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(INIFTA)
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Citación
Torres, María Luz; Fernandez, María José; Dellatorre, Fernando Gaspar; Cortizo, Ana María; Oberti, Tamara Gisela; Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering; Elsevier; Algal Research; 40; 101499; 6-2019; 1-10
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