Artículo
Trypanosoma cruzi Virulence Factors for the Diagnosis of Chagas’ Disease
Chain, Cecilia Yamil
; Pires Souto, Dênio Emanuel; Sbaraglini, Maria Laura
; Labriola, Carlos Alberto
; Daza Millone, Maria Antonieta
; Ramirez, Eduardo Alejandro
; Cisneros, José Sebastián
; Lopez Albizu, Maria Constanza; Scollo, Karenina; Kubota, Lauro T.; Ruiz, Andrés Mariano; Vela, Maria Elena
Fecha de publicación:
09/2019
Editorial:
American Chemical Society
Revista:
Infectious Diseases
ISSN:
2373-8227
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
trans-Sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. The aim of the present study is to assess the possibility of using cruzipain and the catalytic domain of trans-sialidase in a Surface Plasmon Resonance-based immunosensor for the diagnosis of chronic Chagas disease. Immunoassays carried out with canine sera verified that cruzipain allows the detection of anti-Trypanosoma cruzi antibodies whereas recombinant trans-sialidase did not yield specific detections, due to the high dilutions of serum used in the immunoassays that hinder the possibility to sense the specific low titer antibodies. The developed cruzipain-based biosensor, whose price per assay is comparable to a commercial enzyme-linked immunosorbent assay (ELISA), was successfully applied for the rapid quantification of specific antibodies against Trypanosoma cruzi in fresh human sera showing an excellent agreement with ELISA
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Articulos(INIFTA)
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Citación
Chain, Cecilia Yamil; Pires Souto, Dênio Emanuel; Sbaraglini, Maria Laura; Labriola, Carlos Alberto; Daza Millone, Maria Antonieta; et al.; Trypanosoma cruzi Virulence Factors for the Diagnosis of Chagas’ Disease; American Chemical Society; Infectious Diseases; 5; 11; 9-2019; 1813-1819
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