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dc.contributor.author
Macagno, Joana
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dc.contributor.author
Lescano, Maia Raquel
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dc.contributor.author
Berli, Claudio Luis Alberto
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dc.date.available
2020-11-19T12:40:38Z
dc.date.issued
2019-08
dc.identifier.citation
Macagno, Joana; Lescano, Maia Raquel; Berli, Claudio Luis Alberto; Milli-channel array for direct and quick reading of root elongation bioassays; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 178; 8-2019; 51-57
dc.identifier.issn
0147-6513
dc.identifier.uri
http://hdl.handle.net/11336/118634
dc.description.abstract
A novel platform to perform systematic analysis and direct reading of root elongation bioassays is presented. The device was designed to include multiplexed microenvironments for the germination and growth of individual seeds, which allows observation by the naked eye or by optical systems, notably cellphone cameras. Prototypes were fabricated by laser micromachining on a highly transparent material that is fully compatible with biological systems. The effectiveness of the milli-channel array was verified against the conventional system (Petri dish). Lactuca sativa was chosen as a model species and glyphosate as a typical toxic agent. All tests were run according to standardized procedures and data analysis was carried out through different statistical indicators such as the root elongation and germination indexes. Results attained in the milli-channel array were identical to those in Petri dish, with the remarkable benefit that several steps required in the conventional system were avoided, which enormously decreases the operation time and the possibility of experimental errors. Further advantages of the milli-channel array are also reported, such as the capability to achieve live imaging of plant organs growth through a simple experiment. The developed device has been proven to be effective, versatile, easy-to-use, and integrable to cellphones, which naturally provide facilities for data recording, analysis, and networking. These improvements open the route to novel applications of bioassays in the wide field of ecotoxicology and environmental studies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
DIRECT READING
dc.subject
LACTUCA SATIVA
dc.subject
MILLI-CHANNEL ARRAY
dc.subject
PHYTOTOXICITY
dc.subject
ROOT ELONGATION
dc.subject.classification
Otras Ingeniería del Medio Ambiente
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dc.subject.classification
Ingeniería del Medio Ambiente
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Milli-channel array for direct and quick reading of root elongation bioassays
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-18T21:17:04Z
dc.journal.volume
178
dc.journal.pagination
51-57
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Macagno, Joana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Lescano, Maia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
Ecotoxicology and Environmental Safety
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.ecoenv.2019.04.020
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