Artículo
GolS controls the response to gold by the hierarchical induction of Salmonella-specific genes that include a CBA efflux-coding operon
Pontel, Lucas Blas
; Pérez Audero, María Eugenia
; Espariz, Martin
; Checa, Susana Karina
; Soncini, Fernando Carlos
Fecha de publicación:
11/2007
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Molecular Microbiology
ISSN:
0950-382X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Salmonella employs a specific set of proteins that allows it to detect the presence of gold salts in the environment and to mount the appropriate resistance response. This includes a P-type ATPase, GolT, and a small cytoplasmic metal-binding protein, GolB. Their expression is controlled by a MerR-like sensor, GolS, which is highly selective for Au ions. Here, we identify a new GolS-controlled operon named gesABC which codes for a CBA efflux system, and establish its role in Au resistance. GesABC can also mediate drug resistance when induced by Au in a GolS-dependent manner, in a strain deleted in the main drug transporter acrAB. The GolS-controlled transcription of gesABC differs from the other GolS-regulated loci. It is activated by gold, but not induced by copper, even in a strain deleted of the main Cu transporter gene copA, which triggers a substantial GolS-dependent induction of golTS and golB. We demonstrate that the Au-dependent induction of gesABC transcription requires higher GolS levels than for the other members of the gol regulon. This correlates with a divergent GolS operator in the gesABC promoter. We propose that the hierarchical induction within the gol regulon allows Salmonella to cope with Au-contaminated environments.
Palabras clave:
GOLS
,
SALMONELLA
,
CUER
,
COPPER
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Pontel, Lucas Blas; Pérez Audero, María Eugenia; Espariz, Martin; Checa, Susana Karina; Soncini, Fernando Carlos; GolS controls the response to gold by the hierarchical induction of Salmonella-specific genes that include a CBA efflux-coding operon; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 66; 3; 11-2007; 814-825
Compartir
Altmétricas