Artículo
Bedaquiline and linezolid MIC distributions and epidemiological cut-off values for Mycobacterium tuberculosis in the Latin American region
Lopez, Beatriz; Siqueira De Oliveira, Rosangela; Pinhata, Juliana M. W.; Chimara, Erica; Pacheco Ascencio, Edson; Puyén Guerra, Zully M.; Wainmayer, Ingrid; Símboli, Norberto Fabián; Del Granado, Mirtha; Palomino, Juan Carlos; Ritacco, Gloria Viviana
; Martin, Anandi
Fecha de publicación:
02/2019
Editorial:
Oxford University Press
Revista:
Journal of Antimicrobial Chemotherapy
ISSN:
0305-7453
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Objectives To describe the distributions of bedaquiline and linezolid MIC values for the Mycobacterium tuberculosis WT population and to define the corresponding epidemiological cut-offs (ECOFFs) in three Latin American countries. Methods MICs of bedaquiline and linezolid were determined by the resazurin microtitre assay (REMA). In phase 1, interlaboratory reproducibility was assessed using a panel of 10 fully susceptible M. tuberculosis strains. Phase 2 involved MIC determination for 248 clinical isolates from Argentina (n = 58), Brazil (n = 100) and Peru (n = 90) from patients who were treatment-naive for bedaquiline and linezolid. We then determined the ECOFFs for bedaquiline and linezolid by the eyeball method and the ECOFFinder statistical calculator. Results Phase 1: REMA MIC values in the three sites were either identical to each other or differed by one 2-fold dilution from the consensus value with the exception of a single value. Phase 2: The bedaquiline MIC range was 0.0039-0.25 mg/L for pan-susceptible and drug-resistant isolates combined. The linezolid MIC range was 0.062-0.5 mg/L for pan-susceptible isolates and 0.031-4 mg/L for drug-resistant isolates. ECOFFs were 0.125 mg/L for bedaquiline and 0.50 mg/L for linezolid. Conclusions REMA is reproducible and robust for the determination of bedaquiline and linezolid MIC distributions and ECOFF values when applied in laboratories of medium/low-resource countries. We suggest that WT MIC distributions for both drugs should be used as a monitoring tool to control the possible rapid emergence of resistance.
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Articulos(SEDE CENTRAL)
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Articulos de SEDE CENTRAL
Citación
Lopez, Beatriz; Siqueira De Oliveira, Rosangela; Pinhata, Juliana M. W.; Chimara, Erica; Pacheco Ascencio, Edson; et al.; Bedaquiline and linezolid MIC distributions and epidemiological cut-off values for Mycobacterium tuberculosis in the Latin American region; Oxford University Press; Journal of Antimicrobial Chemotherapy; 74; 2; 2-2019; 373-379
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