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dc.contributor.author
Sanchez Vasquez, Estefania
dc.contributor.author
Bronner, Marianne E.
dc.contributor.author
Strobl Mazulla, Pablo Hernan
dc.date.available
2020-11-05T20:09:42Z
dc.date.issued
2019-04
dc.identifier.citation
Sanchez Vasquez, Estefania; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition; Company of Biologists; Development; 146; 7; 4-2019; 1-9
dc.identifier.issn
0950-1991
dc.identifier.uri
http://hdl.handle.net/11336/117727
dc.description.abstract
miR-203 is a tumor-suppressor microRNA with known functions in cancer metastasis. Here, we explore its normal developmental role in the context of neural crest development. During the epithelial-to-mesenchymal transition of neural crest cells to emigrate from the neural tube, miR-203 displays a reciprocal expression pattern with key regulators of neural crest delamination, Phf12 and Snail2, and interacts with their 3′UTRs. We show that ectopic maintenance of miR-203 inhibits neural crest migration in chick, whereas its functional inhibition using a ‘sponge’ vector or morpholinos promotes premature neural crest delamination. Bisulfite sequencing further shows that epigenetic repression of miR-203 is mediated by the de novo DNA methyltransferase DNMT3B, the recruitment of which to regulatory regions on the miR-203 locus is directed by SNAIL2 in a negative-feedback loop. These findings reveal an important role for miR-203 in an epigenetic-microRNA regulatory network that influences the timing of neural crest delamination.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DNA METHYLATION
dc.subject
EMT
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MIGRATION
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MIR-203
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NEURAL CREST
dc.subject
PHF12
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SNAIL2
dc.subject.classification
Biología del Desarrollo
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-05T15:37:40Z
dc.journal.volume
146
dc.journal.number
7
dc.journal.pagination
1-9
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Sanchez Vasquez, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); Argentina
dc.description.fil
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
dc.description.fil
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); Argentina
dc.journal.title
Development
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/lookup/doi/10.1242/dev.171017
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1242/dev.171017
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