Artículo
Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
Mastaglia, Silvina Rosana
; Pellegrini, Gretel Gisela
; Mandalunis, Patricia Mónica; Gonzales Chaves, Macarena Maria Sol
; Friedman, Silvia María; Zeni, Susana Noemi
Fecha de publicación:
10/2006
Editorial:
Elsevier Science Inc
Revista:
Bone
ISSN:
8756-3282
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The present study was carried out to obtain an experimental model of vitamin D (vit D) insufficiency and established osteopenia (experiment 1) to then investigate whether vit D status, i.e. normal or insufficient, interferes with bone mass recovery resulting from bisphosphonate therapy (experiment 2). Rats (n = 40) underwent OVX (n = 32) or a sham operation (n = 8). The first 15 days post-surgery, all groups were kept under fluorescent tube lighting and fed a diet containing 200 IU% vit D (+ D). They were then assigned during an additional 45 days to receive either + D or a diet lacking vit D (- D) and kept under 12 h light/dark cycles using fluorescent or red lighting. Serum 25HOD was significantly lower in - D rats (P < 0.0001). The type of lighting did not induce differences in 25OHD, calcium (sCa), phosphorus (sP), bone alkaline phosphatase (b-AL), CTX, bone density or histology. No osteoid was observed in undecalcified bone sections. Experiment 2 (105 days): rats were fed either + D or - D according to experiment 1 and were treated with either placebo or 16 μg olpadronate (OPD)/100 g rat/week during the last 45 days. Whereas 25HOD was significantly lower (P < 0.0001) in - D/OPD than in + D/OPD rats, no significant differences in sCa, sP, b-AL or CTX were observed. OPD prevented the loss of lumbar spine (LS) and proximal tibia (PT) BMD and the decrease in bone volume (BV/TV) (P < 0.05) and in the number of trabeculae observed in untreated rats. However, + D/OPD animals presented significantly higher values of LS BMD, PT BMD and BV/TV than - D/OPD rats (P < 0.05). No osteoid was observed in undecalcified sections of bone. In summary, this is the first experimental study to provide evidence that differences in vit D status may affect the anticatabolic response to bisphosphonate treatment. However, the molecular mechanism through which vit D insufficiency reduces the effect of the aminobisphosphonate remains to be defined.
Palabras clave:
BISPHOSPHONATES
,
BONE MARKERS
,
OSTEOPENIA
,
RATS
,
VITAMIN D INSUFFICIENCY
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INIGEM)
Articulos de INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Articulos de INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Mastaglia, Silvina Rosana; Pellegrini, Gretel Gisela; Mandalunis, Patricia Mónica; Gonzales Chaves, Macarena Maria Sol; Friedman, Silvia María; et al.; Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats; Elsevier Science Inc; Bone; 39; 4; 10-2006; 837-844
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