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dc.contributor.author
Santa Cruz, R.
dc.contributor.author
Giuliano, S. M.
dc.contributor.author
Gambarotta, M. C.
dc.contributor.author
Morrell, J. M.
dc.contributor.author
Abraham, M. C.
dc.contributor.author
Miragaya, Marcelo
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dc.contributor.author
Carretero, Maria Ignacia
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dc.date.available
2020-11-04T20:56:37Z
dc.date.issued
2016-10
dc.identifier.citation
Santa Cruz, R.; Giuliano, S. M.; Gambarotta, M. C.; Morrell, J. M.; Abraham, M. C.; et al.; Comparison of differents methods of sperm selection of llama raw semen; Elsevier Science; Animal Reproduction Science; 173; 10-2016; 8-12
dc.identifier.issn
0378-4320
dc.identifier.uri
http://hdl.handle.net/11336/117653
dc.description.abstract
The objective of this study was to compare the efficiency of different sperm selection methods applied to the same llama ejaculate. Four treatments were compared: two variants of the swim up technique (with and without seminal plasma), and two different colloids, Androcoll-E-Large and Percoll®. Using electroejaculation, 21 semen samples were obtained from 7 llama males (n = 7, r = 3). The ejaculates were incubated in a solution of 0.1% collagenase, to decrease thread formation, and then split into 4 aliquots: one aliquot was layered over a column of Androcoll-E-Large (SLC) and the second over a column of Percoll (45%). The third aliquot was deposited in a tube with culture medium and was incubated at a 45° angle for 30 min at 37 °C (SU1). The last aliquot was centrifuged to separate the spermatozoa and seminal plasma. The sperm pellet obtained was resuspended, and transferred to a tube with culture medium which was incubated at an angle of 45° for 30 min at 37 °C (SU2). Both aliquots SLC and P showed higher proportions of progressive motility and plasma membrane functionality (p ≤ 0.05) than raw semen. There were no significant differences (p > 0.05) in sperm viability and in normal spermatozoa between raw semen and treatments. Nevertheless, only SLC did not have a significant increase of bent tails. In conclusion SLC centrifugation would be the method of choice for selecting llama spermatozoa.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANDROCOLL
dc.subject
LLAMA
dc.subject
PERCOLL
dc.subject
SPERM SELECTION
dc.subject
SWIM UP
dc.subject.classification
Otras Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Comparison of differents methods of sperm selection of llama raw semen
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-03T16:53:32Z
dc.journal.volume
173
dc.journal.pagination
8-12
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Santa Cruz, R.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Giuliano, S. M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Gambarotta, M. C.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Morrell, J. M.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; Suecia
dc.description.fil
Fil: Abraham, M. C.. Swedish University Of Agricultural Sciences. Faculty Of Veterinary Medicine And Animal Science; Suecia
dc.description.fil
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Carretero, Maria Ignacia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Animal Reproduction Science
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.anireprosci.2016.08.001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378432016303037
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