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Artículo

The ectomycorrhizal basidiomycete Laccaria bicolor releases a secreted β-1,4 endoglucanase that plays a key role in symbiosis development

Zhang, Feng; Anasontzis, George E.; Labourel, Aurore; Champion, Charlotte; Haon, Mireille; Kemppainen, Minna JohannaIcon ; Commun, Carine; Deveau, Aurélie; Pardo, Alejandro GuillermoIcon ; Veneault-Fourrey, Claire; Kohler, Annegret; Rosso, Marie-Noëlle; Henrissat, Bernard; Berrin, Jean-Guy; Martin, Francis
Fecha de publicación: 12/2018
Editorial: Wiley Blackwell Publishing, Inc
Revista: New Phytologist
ISSN: 0028-646X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Micología

Resumen

In ectomycorrhiza, root ingress and colonization of the apoplast by colonizing hyphae is thought to rely mainly on the mechanical force that results from hyphal tip growth, but this could be enhanced by secretion of cell-wall-degrading enzymes, which have not yet been identified. The sole cellulose-binding module (CBM1) encoded in the genome of the ectomycorrhizal Laccaria bicolor is linked to a glycoside hydrolase family 5 (GH5) endoglucanase, LbGH5-CBM1. Here, we characterize LbGH5-CBM1 gene expression and the biochemical properties of its protein product. We also immunolocalized LbGH5-CBM1 by immunofluorescence confocal microscopy in poplar ectomycorrhiza. We show that LbGH5-CBM1 expression is substantially induced in ectomycorrhiza, and RNAi mutants with a decreased LbGH5-CBM1 expression have a lower ability to form ectomycorrhiza, suggesting a key role in symbiosis. Recombinant LbGH5-CBM1 displays its highest activity towards cellulose and galactomannans, but no activity toward L. bicolor cell walls. In situ localization of LbGH5-CBM1 in ectomycorrhiza reveals that the endoglucanase accumulates at the periphery of hyphae forming the Hartig net and the mantle. Our data suggest that the symbiosis-induced endoglucanase LbGH5-CBM1 is an enzymatic effector involved in cell wall remodeling during formation of the Hartig net and is an important determinant for successful symbiotic colonization.
Palabras clave: APOPLASTIC EFFECTOR , CARBOHYDRATE-ACTIVE ENZYMES , CELL-WALL-MODIFYING ENZYMES , CELLULASE , CELLULOSE-BINDING DOMAIN , ENDO-1,4-Β-MANNANASE
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/117651
URL: https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15113
DOI: http://dx.doi.org/10.1111/nph.15113
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Zhang, Feng; Anasontzis, George E.; Labourel, Aurore; Champion, Charlotte; Haon, Mireille; et al.; The ectomycorrhizal basidiomycete Laccaria bicolor releases a secreted β-1,4 endoglucanase that plays a key role in symbiosis development; Wiley Blackwell Publishing, Inc; New Phytologist; 220; 4; 12-2018; 1309-1321
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