Artículo
Photodynamic inactivation of ESKAPE group bacterial pathogens in planktonic and biofilm cultures using metallated porphyrin-doped conjugated polymer nanoparticles
Martinez, Sol Romina
; Ibarra, Luis Exequiel
; Ponzio, Rodrigo Andrés
; Forcone, Maria Virginia; Wendel, Ana Belén
; Chesta, Carlos Alberto
; Spesia, Mariana Belen
; Palacios, Rodrigo Emiliano
Fecha de publicación:
06/2020
Editorial:
American Chemical Society Inc
Revista:
ACS Infectious Diseases
ISSN:
2373-8227
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Photodynamic inactivation (PDI) protocols using photoactive metallated porphyrin-doped conjugated polymer nanoparticles (CPNs) and blue light were developed to eliminate multidrug-resistant pathogens. CPNs-PDI protocols using varying particle concentration and irradiation doses were tested against nine pathogenic bacterial strains including antibiotic-resistant bacteria of the ESKAPE pathogens group. Bactericidal effect was achieved in methicillin-resistant Sthaphylococus aureus strains using low light doses (9.6-14.4 J/cm2); while Gram-negative bacteria required a higher light dose (28.8 J/cm2). Bacteria-CPNs interaction was studied through flow cytometry taking advantage of the intrinsic CPNs fluorescence, demonstrating that CPNs efficiently bind to the bacterial envelope. Finally, the performance of CPNs-PDI was explored in biofilms; good antibiofilm ability and almost complete eradication were observed for S. aureus and Escherichia coli biofilms, respectively, using confocal microscopy. Overall, we demonstrated that CPNs-PDI is an efficient tool not only to kill superbugs as sessile cells but also to disrupt and eradicate biofilms of highly relevant pathogenic bacterial species.
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Articulos (IITEMA)
Articulos de INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Articulos de INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Citación
Martinez, Sol Romina; Ibarra, Luis Exequiel; Ponzio, Rodrigo Andrés; Forcone, Maria Virginia; Wendel, Ana Belén; et al.; Photodynamic inactivation of ESKAPE group bacterial pathogens in planktonic and biofilm cultures using metallated porphyrin-doped conjugated polymer nanoparticles; American Chemical Society Inc; ACS Infectious Diseases; 6; 8; 6-2020; 2202-2213
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