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dc.contributor.author
Barcia, Cristina
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dc.contributor.author
Coehlo, Ana Sofía
dc.contributor.author
Barberis, Sonia Esther
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dc.contributor.author
Veríssimo, Paula
dc.date.available
2020-10-27T17:24:10Z
dc.date.issued
2020-03
dc.identifier.citation
Barcia, Cristina; Coehlo, Ana Sofía; Barberis, Sonia Esther; Veríssimo, Paula; Acaciain peptidase: The first South American pollen peptidase potentially involved in respiratory allergy; Portland Press; Biotechnology and Applied Biochemistry; 67; 2; 3-2020; 224-233
dc.identifier.issn
1470-8744
dc.identifier.uri
http://hdl.handle.net/11336/116951
dc.description.abstract
Acacia caven (Mol.) Molina pollen causes pollinosis in South America. The aim of this work was to isolate, characterize and purify the proteolytic enzymes of A. caven pollen, and study their influence on allergy and asthma diseases. A serial of chromatographic steps was applied to purify the proteolytic extract of A. caven pollen. The purified fractions were partially characterized and then, they were assayed on airway bioactive peptides (Substance P, Vasoactive Intestinal Peptide (VIP) and Bradykinin) and the peptide degradation was visualized by direct protein sequencing. The cellular detachment of an airway-derived epithelial cell line (A-549) was measured by methylene blue binding assay. The degradation of proteins from intercellular junctions (Occludin, Claudin and E-cadherin) was visualized by Western blot. A 75-kDa peptidase from the pollen of Acacia caven, named Acaciain peptidase, was purified and classified as a serine peptidase. Acaciain peptidase degraded bioactive peptides involved in the maintenance and recovery of the bronchomotor tone; it caused cellular detachment of A-549 cell line and degradation of intercellular junction proteins. Our results suggest that Acaciain peptidase can alter the integrity of the epithelium barrier, causing cell permeability, increasing the allergic sensitization and exacerbating the overall bronchoconstrictive effect detected in asthmatic lungs. This novel serine peptidase constitutes a relevant therapeutic target in the treatment of allergic disorders.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Portland Press
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ACACIA CAVEN
dc.subject
ALLERGY
dc.subject
PURIFICATION
dc.subject
SERINE PROTEASE
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Acaciain peptidase: The first South American pollen peptidase potentially involved in respiratory allergy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-22T15:41:10Z
dc.identifier.eissn
0885-4513
dc.journal.volume
67
dc.journal.number
2
dc.journal.pagination
224-233
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Barcia, Cristina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentina
dc.description.fil
Fil: Coehlo, Ana Sofía. Universidad de Coimbra; Portugal
dc.description.fil
Fil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentina
dc.description.fil
Fil: Veríssimo, Paula. Universidad de Coimbra; Portugal
dc.journal.title
Biotechnology and Applied Biochemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/bab.1837
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bab.1837
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