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dc.contributor.author
Sampedro, Maria Florencia
dc.contributor.author
Izaguirre, María Fernanda
dc.contributor.author
Sigot, Valeria
dc.date.available
2020-10-21T12:16:12Z
dc.date.issued
2018-12
dc.identifier.citation
Sampedro, Maria Florencia; Izaguirre, María Fernanda; Sigot, Valeria; E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]; F1000 Research Ltd; F1000Research; 7; 12-2018; 1489-1499
dc.identifier.issn
2046-1402
dc.identifier.uri
http://hdl.handle.net/11336/116222
dc.description.abstract
Background: E-cadherin is the major adhesion receptor in epithelial adherens junctions (AJs). On established epidermis, E-cadherin performs fine-tuned cell-cell contact remodeling to maintain tissue integrity, which is characterized by modulation of cell shape, size and packing density. In zebrafish, the organization and distribution of E-cadherin in AJs during embryonic epidermis development remain scarcely described. Methods: Combining classical immunofluorescence, deconvolution microscopy and 3D-segmentation of AJs in epithelial cells, a quantitative approach was implemented to assess the spatial and temporal distribution of E-cadherin across zebrafish epidermis between 24 and 72 hpf. Results: increasing levels of E-cadh protein parallel higher cell density and the appearance of hexagonal cells in the enveloping layer (EVL) as well as the establishments of new cell-cell contacts in the epidermal basal layer (EBL), being significantly between 31 and 48 hpf. Conclusions: Increasing levels of E-cadherin in AJs correlates with extensive changes in cell morphology towards hexagonal packing during the epidermis morphogenesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
F1000 Research Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
3D SEGMENTATION
dc.subject
DECONVOLUTION
dc.subject
E-CADHERIN; ADHERENS JUNCTIONS; ZEBRAFISH EPIDERMIS; ENVELOPING LAYER (EVL)
dc.subject
EPIDERMIS BASAL LAYER (EBL)
dc.subject.classification
Biología del Desarrollo
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
E-cadherin expression pattern during zebrafish embryonic epidermis development [version 3; referees: 3 approved]
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-10-19T16:17:15Z
dc.journal.volume
7
dc.journal.pagination
1489-1499
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
dc.description.fil
Fil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
dc.description.fil
Fil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
dc.journal.title
F1000Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://f1000research.com/articles/7-1489/v3
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.12688/f1000research.15932.3
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