Artículo
Glucosamine-P and rhodococcal ADP-glucose pyrophosphorylases: A hint to (re)discover (actino)bacterial amino sugar metabolism
Cereijo, Antonela Estefanía
; Alvarez, Hector Manuel
; Iglesias, Alberto Alvaro
; Asención Diez, Matías Damián
Fecha de publicación:
09/2020
Editorial:
Elsevier France-editions Scientifiques Medicales Elsevier
Revista:
Biochimie
ISSN:
0300-9084
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Glycogen was described as a temporal storage molecule in rhodococci, interconnecting lipids and carbon availability. The Rhodococcus jostii ADP-glucose pyrophosphorylase (ADP-GlcPPase) kinetic and regulatory properties support this role. Curiously, the enzyme uses glucosamine-1P as alternative substrate. Herein, we report the in-depth study of glucosamine-1P activity and its regulation in two rhodocoocal ADP-GlcPPases, finding that glucosamine-6P (representing a metabolic carbon/nitrogen node) is a critical activator, then reinforcing the role of glycogen as an ?intermediary metabolite? in rhodococci. Glucosamine-1P activity in rhodococcal ADP-GlcPPases responds to activation by metabolites improving their catalytic performance, strongly suggesting its metabolic feasibility. This work supports a scenario for new molecules/metabolites discovery and hypothesizes on evolutionary mechanisms underlying enzyme promiscuity opening novel metabolic features in (actino)bacteria.
Palabras clave:
GLUCOSAMINE-6P
,
GLUCOSAMINE-1P
,
ALLOSTERISM
,
GLYCOGEN
,
TRIACYLGLYCEROL
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Cereijo, Antonela Estefanía; Alvarez, Hector Manuel; Iglesias, Alberto Alvaro; Asención Diez, Matías Damián; Glucosamine-P and rhodococcal ADP-glucose pyrophosphorylases: A hint to (re)discover (actino)bacterial amino sugar metabolism; Elsevier France-editions Scientifiques Medicales Elsevier; Biochimie; 176; 9-2020; 158-161
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