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dc.contributor.author
Sacco, Sofía Clara
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Velazquez, Natalia Soledad
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Brennan, María Ana
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Beccaría, Camila
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Baravalle, Celina
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Pereyra, Elizabet Amanda Lorena
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Monecke, Stefan
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Calvinho, Luis F.
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Dallard, Bibiana Elisabet
dc.date.available
2020-10-19T14:12:05Z
dc.date.issued
2020-05
dc.identifier.citation
Sacco, Sofía Clara; Velazquez, Natalia Soledad; Brennan, María Ana; Beccaría, Camila; Baravalle, Celina; et al.; Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 142; 5-2020; 1-12
dc.identifier.issn
0882-4010
dc.identifier.uri
http://hdl.handle.net/11336/116102
dc.description.abstract
The aim of this study was to evaluate and compare the ability to adhere/internalize, persist, and induce damage in mammary epithelial cells (MAC-T) of two Staphylococcus aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG). Also, the phagocytic and bactericidal capacity induced after the interaction between macrophages, isolated from mammary secretion, of both S. aureus strains was evaluated. Two isolates (designated 806 and 5011) from bovine intramammary infection (IMI) harboring genes involved in adherence and biofilm production, belonging to different capsular polysaccharide (CP) type, accessory gene regulator (agr) group, pulsotype (PT) and sequence type/clonal complex (ST/CC). Strains 806 and 5011 were associated with low (nonpersistent-NP) and high (persistent-P) adaptation to the MG, respectively. Strain 5011 (P), agr group I, cap8 positive and strong biofilm producer showed higher capacity to adhere/internalize in MAC-T compared with strain 806 (NP), characterized as agr group II, cap5 positive and weak biofilm producer. Strain 5011(P) could be recovered from MAC-T lysates up to 72 h pi; while strain 806 (NP) could be recovered only at 4 h pi. Strain 5011 (P) showed greater capacity to induce apoptosis compared with strain 806 (NP) at 4, 24 and 48 h pi. Macrophages infected with strain 5011 (P) showed a greater phagocytic capacity and higher percentage of intracellular reactive oxygen species (ROS) production than strain 806 (NP). No viable bacteria were isolated from macrophages lysates stimulated with any of the S. aureus strains at 2, 4, 8 and 24 h pi. The knowledge of the molecular profile of the S. aureus strains causing bovine mastitis in a herd could become a tool to expose the most prevalent virulence gene patterns and advance in the elucidation of the pathogenesis of chronic mastitis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Ltd - Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BOVINE MASTITIS
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DAMAGE
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MACROPHAGES
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MAMMARY EPITHELIAL CELLS
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PERSISTENCE
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STAPHYLOCOCCUS AUREUS
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-25T19:06:44Z
dc.journal.volume
142
dc.journal.pagination
1-12
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Sacco, Sofía Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
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Fil: Velazquez, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
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Fil: Brennan, María Ana. Universidad Nacional del Litoral; Argentina
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Fil: Beccaría, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
dc.description.fil
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
dc.description.fil
Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
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Fil: Monecke, Stefan. Alere Technologies Gmbh; Alemania
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Fil: Calvinho, Luis F.. Technische Universität Dresden; Alemania
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Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
dc.journal.title
Microbial Pathogenesis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0882401019318546
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.micpath.2020.104017
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