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dc.contributor.author
Anchordoquy, Juan Mateo  
dc.contributor.author
Anchordoquy, Juan Patricio  
dc.contributor.author
Sirini, Matias Angel  
dc.contributor.author
Picco, Sebastian Julio  
dc.contributor.author
Peral Garcia, Pilar  
dc.contributor.author
Furnus, Cecilia Cristina  
dc.date.available
2017-01-18T21:00:02Z  
dc.date.issued
2014-10  
dc.identifier.citation
Anchordoquy, Juan Mateo; Anchordoquy, Juan Patricio; Sirini, Matias Angel; Picco, Sebastian Julio; Peral Garcia, Pilar; et al.; The importance of having zinc during in vitro maturation of cattle Cumulus–Oocyte complex: role of Cumulus cells; Wiley VCH Verlag; Reproduction In Domestic Animals; 49; 5; 10-2014; 865–874  
dc.identifier.issn
0936–6768  
dc.identifier.uri
http://hdl.handle.net/11336/11605  
dc.description.abstract
The aim of this study was to investigate the influence of zinc (Zn) on the health of cumulus–oocyte complex (COC) during in vitro maturation (IVM). Experiments were designed to evaluate the effect of Zn added to IVM medium on: DNA integrity, apoptosis, cumulus expansion and superoxide dismutase (SOD) activity of cumulus cells (CC). Also, role of CC on Zn transport during IVM was evaluated on oocyte developmental capacity. DNA damage and early apoptosis were higher in CC matured with 0 μg/ml Zn compared with 0.7, 1.1 and 1.5 μg/ml Zn (p < 0.05). Cumulus expansion did not show differences in COC matured with or without Zn supplementation (p > 0.05). Superoxide dismutase activity was higher in COC matured with 1.5 μg/ml Zn than with 0 μg/ml Zn (p < 0.05). Cleavage and blastocyst rates were recorded after IVM in three maturation systems: intact COCs, denuded oocytes with cumulus cells monolayer (DO + CC) and denuded oocytes (DO). Cleavage rates were similar when COC, DO + CC or DO were matured with 1.5 μg/ml Zn compared with control group (p > 0.05). Blastocyst rates were significantly higher in COC than in DO + CC and DO with the addition of 1.5 μg/ml Zn during IVM (p < 0.01). Blastocyst quality was enhanced in COC and DO + CC compared with DO when Zn was added to IVM medium (p < 0.001). The results of this study indicate that Zn supplementation to IVM medium (i) decreased DNA damage and apoptosis in CC; (ii) increased SOD activity in CC; (iii) did not modify cumulus expansion and cleavage rates after in vitro fertilization; (iv) improved subsequent embryo development up to blastocyst stage; and (v) enhanced blastocyst quality when CC were present either in intact COC or in coculture during IVM.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley VCH Verlag  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
In Vitro Maturation  
dc.subject
Zinc  
dc.subject
Embryo Development  
dc.subject
Cumulus Cells  
dc.subject.classification
Ciencias Veterinarias  
dc.subject.classification
Ciencias Veterinarias  
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CIENCIAS AGRÍCOLAS  
dc.title
The importance of having zinc during in vitro maturation of cattle Cumulus–Oocyte complex: role of Cumulus cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-01-17T19:06:13Z  
dc.journal.volume
49  
dc.journal.number
5  
dc.journal.pagination
865–874  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlin  
dc.description.fil
Fil: Anchordoquy, Juan Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Catedra de Fisiologia; Argentina  
dc.description.fil
Fil: Anchordoquy, Juan Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Catedra de Fisiologia; Argentina  
dc.description.fil
Fil: Sirini, Matias Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Catedra de Fisiologia; Argentina  
dc.description.fil
Fil: Picco, Sebastian Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Catedra de Fisiologia; Argentina  
dc.description.fil
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina  
dc.description.fil
Fil: Furnus, Cecilia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina  
dc.journal.title
Reproduction In Domestic Animals  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/rda.12385  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12385/abstract