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dc.contributor.author
Giovambattista, Guillermo
dc.contributor.author
Takeshima, Shin Nosuke
dc.contributor.author
Ripoli, María Verónica
dc.contributor.author
Matsumoto, Yuki
dc.contributor.author
Alvarez Franco, Luz Angela
dc.contributor.author
Saito, Hideki
dc.contributor.author
Onuma, Misao
dc.contributor.author
Aida, Yoko
dc.date.available
2017-01-18T20:31:19Z
dc.date.issued
2013-04
dc.identifier.citation
Giovambattista, Guillermo; Takeshima, Shin Nosuke; Ripoli, María Verónica; Matsumoto, Yuki; Alvarez Franco, Luz Angela; et al.; Characterization of bovine MHC DRB3 diversity in Latin American Creole cattle breeds; Elsevier Science; Gene; 519; 1; 4-2013; 150-158
dc.identifier.issn
0378-1119
dc.identifier.uri
http://hdl.handle.net/11336/11597
dc.description.abstract
In cattle, bovine leukocyte antigens (BoLAs) have been extensively used as markers for diseases and immunological traits. However, none of the highly adapted Latin American Creole breeds have been characterized for BoLA gene polymorphism by high resolution typing methods. In this work, we sequenced exon 2 of the BoLA class II DRB3 gene from 179 cattle (113 Bolivian Yacumeño cattle and 66 Colombian Hartón del Valle cattle breeds) using a polymerase chain reaction sequence-based typing (PCR-SBT) method. We identified 36 previously reported alleles and three novel alleles. Thirty-five (32 reported and three new) and 24 alleles (22 reported and two new) were detected in Yacumeño and Hartón del Valle breeds, respectively. Interestingly, Latin American Creole cattle showed a high degree of gene diversity despite their small population sizes, and 10 alleles including three new alleles were found only in these two Creole breeds. We next compared the degree of genetic variability at the population and sequence levels and the genetic distance in the two breeds with those previously reported in five other breeds: Holstein, Japanese Shorthorn, Japanese Black, Jersey, and Hanwoo. Both Creole breeds presented gene diversity higher than 0.90, a nucleotide diversity higher than 0.07, and mean number of pairwise differences higher than 19, indicating that Creole cattle had similar genetic diversity at BoLA-DRB3 to the other breeds. A neutrality test showed that the high degree of genetic variability may be maintained by balancing selection. The FST index and the exact G test showed significant differences across all cattle populations (FST = 0.0478; p < 0.001). Results from the principal components analysis and the phylogenetic tree showed that Yacumeño and Hartón del Valle breeds were closely related to each other. Collectively, our results suggest that the high level of genetic diversity could be explained by the multiple origins of the Creole germplasm (European, African and Indicus), and this diversity might be maintained by balancing selection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Bola-Drb3 Polymorphism
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Major Histocompatibility Complex Class Ii
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Latin American Creole Cattle
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Sequence-Based Typing
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Yacumeño
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Hartón del Valle
dc.subject.classification
Producción Animal y Lechería
dc.subject.classification
Producción Animal y Lechería
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Characterization of bovine MHC DRB3 diversity in Latin American Creole cattle breeds
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-01-17T19:01:53Z
dc.journal.volume
519
dc.journal.number
1
dc.journal.pagination
150-158
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Takeshima, Shin Nosuke. Riken. Viral Infectious Diseases Unit; Japón
dc.description.fil
Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Matsumoto, Yuki. Riken. Viral Infectious Diseases Unit; Japón
dc.description.fil
Fil: Alvarez Franco, Luz Angela. Universidad Nacional de Colombia; Colombia
dc.description.fil
Fil: Saito, Hideki. Uganda Animal Disease Control Project at Makerere University; Uganda
dc.description.fil
Fil: Onuma, Misao. Riken. Viral Infectious Diseases Unit; Japón
dc.description.fil
Fil: Aida, Yoko. Riken. Viral Infectious Diseases Unit; Japón
dc.journal.title
Gene
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.gene.2013.01.002
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S037811191300019X


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