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dc.contributor.author
Camara, Maria de Los Milagros
dc.contributor.author
Bouvier, Leon Alberto
dc.contributor.author
Miranda, Mariana Reneé
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Reigada, Chantal
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Pereira, Claudio Alejandro
dc.date.available
2017-01-18T18:51:57Z
dc.date.issued
2013-03
dc.identifier.citation
Camara, Maria de Los Milagros; Bouvier, Leon Alberto; Miranda, Mariana Reneé; Reigada, Chantal; Pereira, Claudio Alejandro; Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites; Folia Parasitologica; Folia Parasitologica; 60; 5; 3-2013; 406-410
dc.identifier.issn
0015-5683
dc.identifier.uri
http://hdl.handle.net/11336/11566
dc.description.abstract
Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin-resistant cell line.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Folia Parasitologica
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Chagas Disease
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Metacyclogenesis
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Parasite Differentiation
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Intracellular Epimastigotes
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-24T17:22:13Z
dc.journal.volume
60
dc.journal.number
5
dc.journal.pagination
406-410
dc.journal.pais
República Checa
dc.description.fil
Fil: Camara, Maria de Los Milagros. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Bouvier, Leon Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Reigada, Chantal. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
dc.journal.title
Folia Parasitologica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://folia.paru.cas.cz/artkey/fol-201305-0003_Laboratory_techniques_to_obtain_different_forms_of_Trypanosoma_cruzi_applications_to_wild-type_and_genetically.php
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.14411/fp.2013.042
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