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dc.contributor.author
Contreras Molina, Maria Isabel
dc.contributor.author
Otegui, Marisa
dc.contributor.author
Petruccelli, Silvana
dc.date.available
2020-10-01T18:03:30Z
dc.date.issued
2006-06
dc.identifier.citation
Contreras Molina, Maria Isabel; Otegui, Marisa; Petruccelli, Silvana; Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 9; 3; 6-2006; 1-5
dc.identifier.issn
0717-3458
dc.identifier.uri
http://hdl.handle.net/11336/115294
dc.description.abstract
Storage proteins are transported to a special storage compartments in seeds by Golgi dependent or independent pathways depending on the plant species. The aim of this work was to study the sunflower storage protein transport pathway and to identify component of the sorting machinery. Immature sunflower seeds were analyzed by subcellular fractionation (using percoll and sucrose gradients) and electron microscopy. The vesicles isolated with percoll, have precursors of 11S globulins, a-TIP, d-TIP, BiP, and two bands that have homology to the pumpkin vacuolar sorting receptor PV72. Sucrose isolated vesicles have the same composition than percoll ones, except for the lack of BiP and the presence of only one protein that has reactivity with pea VSR BP80. Electronic micrographies of developing seeds show that the formation of electron dense aggregates starts in the endoplasmic reticulum, and that these aggregates are very abundant in the trans-Golgi apparatus, where release of dense vesicles happens. These vesicles contain a homolog of PV72 in their membranes. Storage proteins are also detected in multivesicular bodies whose membranes have reactivity with PV72 serum. All these results indicated that sunflower storage proteins are transported to protein storage vacuoles by a Golgi dependent pathway in a process in which homologous of PV72 are involved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pontificia Universidad Católica de Valparaíso
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Developing sunflower seeds
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Storage proteins
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Transport pathway
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Vacuolar sorting
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Vacuolar sorting receptor
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-25T16:53:55Z
dc.journal.volume
9
dc.journal.number
3
dc.journal.pagination
1-5
dc.journal.pais
Chile
dc.journal.ciudad
Valparaiso
dc.description.fil
Fil: Contreras Molina, Maria Isabel. Michigan State University; Estados Unidos
dc.description.fil
Fil: Otegui, Marisa. University of Wisconsin; Estados Unidos
dc.description.fil
Fil: Petruccelli, Silvana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
dc.journal.title
Electronic Journal of Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://tinyurl.com/yyqqvxf5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2225/vol9-issue3-fulltext-21
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