Strategies to develop therapeutic N- and O-hyperglycosylated proteins

Abstract

Glycoengineering by N- and/or O-hyperglycosylation represents a procedure to introduce potential sitesfor adding N- and/or O-glycosyl structures to proteins with the aim of producing biotherapeutics withimproved pharmacodynamic and pharmacokinetic properties. In this chapter, a detailed description of thesteps routinely performed to generate new proteins having high content of N- and/or O-glycosyl moietiesis carried out. The rational strategy involves the initial stage of designing N- and/or O-hyperglycosylatedmuteins to be expressed by mammalian cells and includes the upstream and downstream processing stagesnecessary to develop hyperglycosylated versions of the proteins of interest with the purpose of beginningthe long road toward producing biobetters.