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dc.contributor.author
Larsen, Alejandra
dc.contributor.author
González, Ester Teresa
dc.contributor.author
Serena, Maria Soledad
dc.contributor.author
Echeverria, Maria Gabriela
dc.contributor.author
Mortola, Eduardo
dc.date.available
2017-01-17T20:53:57Z
dc.date.issued
2013-06
dc.identifier.citation
Larsen, Alejandra; González, Ester Teresa; Serena, Maria Soledad; Echeverria, Maria Gabriela; Mortola, Eduardo; Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease; Humana Press; Molecular Biotechnology; 54; 2; 6-2013; 475–483
dc.identifier.issn
1073-6085
dc.identifier.uri
http://hdl.handle.net/11336/11513
dc.description.abstract
Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bovine Leukemia Virus
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Immunodetection
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Baculovirus System
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Recombinant P24 Protein
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Immunoblotting Assay
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Expression of p24 gag protein of Bovine Leukemia Virus in insect cells and its use in immunodetection of the disease
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-01-17T19:05:00Z
dc.identifier.eissn
1559-0305
dc.journal.volume
54
dc.journal.number
2
dc.journal.pagination
475–483
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Larsen, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: González, Ester Teresa. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
dc.description.fil
Fil: Serena, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
dc.description.fil
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
dc.description.fil
Fil: Mortola, Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.journal.title
Molecular Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12033-012-9587-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs12033-012-9587-7
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