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dc.contributor.author
Freire, Miguel Angel
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dc.date.available
2020-09-28T21:35:25Z
dc.date.issued
2020-10
dc.identifier.citation
Freire, Miguel Angel; Viral silencing suppressors and cellular proteins partner with plant RRP6-like exoribonucleases; Springer; Virus Genes; 56; 5; 10-2020; 621-631
dc.identifier.issn
0920-8569
dc.identifier.uri
http://hdl.handle.net/11336/115040
dc.description.abstract
RNA silencing and RNA decay are functionally interlaced, regulate gene expression and play a pivotal role in antiviral responses. As a counter defensive strategy, many plant and mammalian viruses encode suppressors which interfere with both mechanisms. However, the protein interactions that connect these pathways remain elusive. Previous work reported that RNA silencing suppressors from different potyviruses, together with translation initiation factors EIF(iso)4E, interacted with the C-terminal region of the tobacco exoribonuclease RRP6-Like 2, a component of the RNA decay exosome complex. Here, we investigate whether other viral silencing suppressors and cellular proteins might also bind RRP6-Like exoribonucleases. A candidate search approach based on yeast-two hybrid protein interaction assays showed that three other unrelated viral suppressors, two from plant viruses and one from a mammalian virus, bound the C-terminus of the tobacco RRP6-Like 2, the full-length of the Arabidopsis RRP6L1 protein and its C-terminal region. In addition, RRP6-Like proteins were found to interact with members of the cellular double-stranded RNA binding protein (DRB) family involved in RNA silencing. The C-terminal regions of RRP6L proteins are engaged in homotypic and heterotypic interactions and were predicted to be disordered. Collectively, these results suggest a protein interaction network that connects components of RNA decay and RNA silencing that is targeted by viral silencing suppressors.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DRB PROTEINS
dc.subject
HEAT SHOCK PROTEIN
dc.subject
RNA DECAY
dc.subject
RNASE H-LIKE
dc.subject
TRANSLATION INITIATION FACTOR EIF(ISO)4E
dc.subject
VIRAL SILENCING SUPPRESSOR
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Viral silencing suppressors and cellular proteins partner with plant RRP6-like exoribonucleases
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-21T14:47:35Z
dc.identifier.eissn
1572-994X
dc.journal.volume
56
dc.journal.number
5
dc.journal.pagination
621-631
dc.journal.pais
Alemania
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dc.description.fil
Fil: Freire, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.journal.title
Virus Genes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11262-020-01775-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11262-020-01775-z
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