Artículo
Artesunate exerts specific cytotoxicity in retinoblastoma cells via CD71
Fecha de publicación:
2013
Editorial:
Spandidos Publ Ltd
Revista:
Oncology Reports
ISSN:
1021-335X
e-ISSN:
1791-2431
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Retinoblastoma (RB) is an intraocular cancer that affects young children. There is an ongoing effort to find new agents for RB management that are effective, specific and with few side-effects. In the present study, we tested artesunate (ART), a synthetic derivative from the herbal drug artemisinin, used in the clinic for the treatment of malaria. We analyzed ART cytotoxicity in an RB cell line (RB-Y79) and in a retinal epithelial cell line (hTERT-RPE1) by flow cytometric analysis (FCM). We related the effect of ART to the expression of transferrin receptor 1 (TfR-1, also known as CD71) by knocking down CD71 with RNAi and analyzing cell cycle variables by FCM. We found that the cytotoxic action of ART is specific for RB cells in a dose-dependent manner, with low toxicity in normal retina cells. ART is more effective in RB than carboplatin with a markedly strong cytotoxic effect on carboplatin-resistant RB cells. RB had higher CD71 levels at the membrane compared to normal retinal cells. We showed that ART internalization in RB cells is dependent upon the expression of the CD71. In addition, ART blocked the cell cycle progression at the G1 phase, even at low doses, and decreased the proportion of RB cells in the S phase. In conclusion, we showed that ART is a promising drug exhibiting high selective cytotoxicity even against multidrug-resistant RB cells. Thus, we suggest that ART could be used in the treatment of RB.
Palabras clave:
Retinoblastoma
,
Artemisin
,
Transferrin Receptor
,
Carboplatin Resistant
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INICSA)
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Citación
Zhao, Fei; Wang, Han; Kunda, Patricia Elena; Chen, Xuemei; Liu, Qiu Ling; et al.; Artesunate exerts specific cytotoxicity in retinoblastoma cells via CD71; Spandidos Publ Ltd; Oncology Reports; 30; -1-2013; 1473-1482
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