Artículo
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
Figueroa Espinosa, Roque Arnulfo
; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela
; Radice, Marcela Alejandra
; Vay, Carlos Alberto; Barrios, Rubén; Gutkind, Gabriel Osvaldo
; Di Conza, José Alejandro
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Fecha de publicación:
05/2018
Editorial:
Elsevier Science
Revista:
Journal of Microbiological Methods
ISSN:
0167-7012
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.
Palabras clave:
CMY-2
,
ESCHERICHIA COLI
,
MALDI-TOF
,
Β-LACTAMASE DETECTION
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Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-28
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