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dc.contributor.author
Busso, Carlos Alberto  
dc.contributor.author
Torres, Yanina Alejandra  
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Ithurrart, Leticia Soledad  
dc.contributor.author
Richards, J. H.  
dc.date.available
2017-01-16T19:52:54Z  
dc.date.issued
2015-07  
dc.identifier.citation
Busso, Carlos Alberto; Torres, Yanina Alejandra; Ithurrart, Leticia Soledad; Richards, J. H.; The TTC-technique might not appropriately test the physiological stage of plant tissues; Springer; Russian Journal Of Plant Physiology; 62; 4; 7-2015; 551-556  
dc.identifier.issn
1021-4437  
dc.identifier.issn
1608-3407  
dc.identifier.uri
http://hdl.handle.net/11336/11421  
dc.description.abstract
The 2,3,5triphenyltetrazolium chloride (TTC) technique has been used during decades to distin guish between dead and alive tissues of perennial grasses. This technique did not consider, however, that dormant (i.e., viable) tissues could exist within those erroneously considered dead tissues, thus being unable to report the true physiological stage of those plant tissues. Development of a procedure able to distinguish between metabol ically active or dormant or dead tissues is then critical. This study developed a procedure to classify plant tissues of perennial grasses (Poa ligularis (Nees ex Steud.), Nassella longiglumis (Phil.) Barkworth, Amelichloa ambigua (Speg.) Arriaga & Barkworth, and Piptochaetium napostaense (Speg.) Hack.) in a more appropriate physiologi cal stage (i.e., metabolically active, dormant or dead) than the traditional TTCtechnique. Perennial grass seeds or meristematic buds were immersed in a TTC solution to obtain metabolically active (red or pink staining) or unstained (either dormant or dead) tissues. TTCunstained tissues were placed in Evans Blue solution to sepa rate dormant from dead tissues. The combination of TTC and Evans Blue techniques allowed the separation of metabolically active, dormant or dead tissues. Use of Evans Blue on TTCunstained seeds and buds allowed to determine that some of these tissues were not dead but dormant (i.e., viable). Among the TTCunstained tissues between 2 to 35% of total grass seeds and from 19.5 to 42% of all evaluated buds were dormant (viable and poten tially able to growout) but not dead. Combination of TTC and Evans Blue techniques allowed a better classifi cation of the physiological stages of plant tissues (metabolically active, dormant or dead) than the conventional TTC test.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Poa Ligularis  
dc.subject
Nassella Longiglumis  
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Amelichloa Ambigua  
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Piptochaetium Napostaense  
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Seeds  
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Axillary Buds  
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Dead an Alive Tissues  
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Ttc-Technique  
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Evans Blue Technique  
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Ecología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
The TTC-technique might not appropriately test the physiological stage of plant tissues  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-01-13T19:21:13Z  
dc.journal.volume
62  
dc.journal.number
4  
dc.journal.pagination
551-556  
dc.journal.pais
Rusia  
dc.journal.ciudad
Kiev  
dc.description.fil
Fil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina  
dc.description.fil
Fil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina  
dc.description.fil
Fil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina  
dc.description.fil
Fil: Richards, J. H.. University of California; Estados Unidos  
dc.journal.title
Russian Journal Of Plant Physiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1134%2FS1021443715040068  
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1134/S1021443715040068