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dc.contributor.author
Dallard, Bibiana Elisabet
dc.contributor.author
Baravalle, Celina
dc.contributor.author
Ortega, Hugo Hector
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Ruffino, Valeria
dc.contributor.author
Heffel, Silvia
dc.contributor.author
Calvinho, Luis Fernando
dc.date.available
2020-09-16T14:52:47Z
dc.date.issued
2008-05
dc.identifier.citation
Dallard, Bibiana Elisabet; Baravalle, Celina; Ortega, Hugo Hector; Ruffino, Valeria; Heffel, Silvia; et al.; Effect of a biological response modifier on cellular death mechanisms at drying off; Cambridge University Press; Journal of Dairy Research; 75; 2; 5-2008; 167-175
dc.identifier.issn
0022-0299
dc.identifier.uri
http://hdl.handle.net/11336/114117
dc.description.abstract
Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objective of this study was to describe the effects of a single intramammary infusion of a lipopolysaccharide (LPS)-based biological response modifier (BRM) on cellular death mechanism in uninfected and Staphylococcus aureus-infected bovine mammary glands during involution. Three groups of 12 cows, each one including 6 Staph. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax at every observation period. In addition, BRM produced a significant increase of immunostained stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of involution compared with placebo-treated quarters and a significant decrease in percentages of terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3 and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining, was increased in epithelial and stromal cells from Staph. aureus-infected compared with uninfected quarters at every observation period. These results provide new insights into the mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell proliferation during mammary involution.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
APOPTOSIS
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BIOLOGICAL RESPONSE MODIFIER
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MAMMARY GLAND INVOLUTION
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STAPHYLOCOCCUS AUREUS
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Effect of a biological response modifier on cellular death mechanisms at drying off
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-03T19:23:37Z
dc.journal.volume
75
dc.journal.number
2
dc.journal.pagination
167-175
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
dc.description.fil
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
dc.description.fil
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
dc.description.fil
Fil: Ruffino, Valeria. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Heffel, Silvia. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Calvinho, Luis Fernando. Universidad Nacional del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
dc.journal.title
Journal of Dairy Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.cambridge.org/action/displayJournal?jid=DAR
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1017/S0022029908003142