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Artículo

Functional thioredoxin reductase from pathogenic and free-living Leptospira spp.

Sasoni, NataliaIcon ; Iglesias, Alberto AlvaroIcon ; Guerrero, Sergio AdrianIcon ; Arias, Diego GustavoIcon
Fecha de publicación: 05/2016
Editorial: Elsevier Science Inc
Revista: Free Radical Biology and Medicine
ISSN: 0891-5849
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Low molecular mass thiols and antioxidant enzymes have essential functions to detoxify reactive oxygen and nitrogen species maintaining cellular redox balance. The metabolic pathways for redox homeostasis in pathogenic (Leptospira interrogans) and free-living (Leptospira biflexa) leptospires species were not functionally characterized. We performed biochemical studies on recombinantly produced proteins to in depth analyze kinetic and structural properties of thioredoxin reductase (LinTrxR) and thioredoxin (LinTrx) from L. interrogans, and two TrxRs (LbiTrxR1 and LbiTrxR2) from L. biflexa. All the TrxRs werecharacterized as homodimeric flavoproteins, with LinTrxR and LbiTrxR1 catalyzing the NADPH dependent reduction of LinTrx and DTNB. The thioredoxin system from L. interrogans was able to use glutathione disulfide, lipoamide disulfide, cystine and bisγ-glutamyl cysteine and homologous peroxiredoxin as substrates. Classic TrxR activity of LinTrxR2 had not been evidenced in vitro, but recombinant Escherichia coli cells overexpressing LbiTrxR2 showed high tolerance to oxidative stress. The enzymatic systemsherein characterized could play a key role for the maintenance of redox homeostasis and the function of defense mechanisms against reactive oxidant species in Leptospira spp. Our results contribute to the general knowledge about redox biochemistry in these bacteria, positioning TrxR as a critical molecular target for the development of new anti-leptospiral drugs.
Palabras clave: LEPTOSPIRA , THIOREDOXIN REDUCTASE , PEROXIREDOXIN , REDOX METABOLISM
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/113860
URL: http://www.sciencedirect.com/science/article/pii/S0891584916302441
DOI: http://dx.doi.org/10.1016/j.freeradbiomed.2016.05.008.
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Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Sasoni, Natalia; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; Arias, Diego Gustavo; Functional thioredoxin reductase from pathogenic and free-living Leptospira spp.; Elsevier Science Inc; Free Radical Biology and Medicine; 97; 5-2016; 1-13
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