Artículo
Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor Α2 in colorectal cells and tumor tissues with different metastatic potential
Serafín, V.; Valverde, A.; Martínez García, G.; Martínez Periñán, E.; Comba, Fausto Nahuel
; Garranzo Asensio, M.; Barderas, R.; Yáñez Sedeño, P.; Campuzano, S.; Pingarrón, J. M.
Fecha de publicación:
01/04/2019
Editorial:
Elsevier Science Sa
Revista:
Sensors and Actuators B: Chemical
ISSN:
0925-4005
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In this work, the development of the first integrated electrochemical immunosensor for the determination of the IL-13Rα2 is reported. The strategy involves the immobilization of a biotinylated capture antibody onto streptavidin-modified screen-printed electrodes through grafting with p-aminobezoic acid (p-ABA) and further activation using EDC/Sulfo-NHS chemistry. A hybrid nanomaterial composed of multiwalled carbon nanotubes (MWCNTs) and Graphene Quantum Dots (GQDs) was used as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the system H 2 O 2 /hydroquinone (HQ) achieved a linear calibration plot ranging from 2.7 to 100 ng mL −1 IL-13sRα2, with a LOD value of 0.8 ng mL −1 . The immunosensor showed an excellent selectivity and was successfully applied to the determination of the target receptor directly in small amounts of raw cellular lysates and extracts of paraffin-embedded tissues from patients diagnosed with colorectal cancer at different stages.
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Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Serafín, V.; Valverde, A.; Martínez García, G.; Martínez Periñán, E.; Comba, Fausto Nahuel; et al.; Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor Α2 in colorectal cells and tumor tissues with different metastatic potential; Elsevier Science Sa; Sensors and Actuators B: Chemical; 284; 01-4-2019; 711-722
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