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Artículo

Identification of novel vaccine candidates for Chagas’ disease by immunization with sequential fractions of a trypomastigote cDNA expression library

Alba Soto, Catalina DirneyIcon ; Tekiel, Valeria SoniaIcon ; González, Stella MarisIcon ; Postan, MiriamIcon ; Sanchez, Daniel OscarIcon
Fecha de publicación: 02/2009
Editorial: Elsevier
Revista: Vaccine
ISSN: 0264-410X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología; Bioquímica y Biología Molecular; Inmunología

Resumen

The protozoan Trypanosoma cruzi is the etiological agent of Chagas´ disease, a major chronic infection in Latin America. Currently, there are neither effective drugs nor vaccines for the treatment or prevention of the disease. Several T. cruzi surface antigens are being tested as vaccines but none of them proved to be completely protective, probably because they represent only a limited repertoire of all the possible T. cruzi target molecules. Taking into account that the trypomastigote stage of the parasite must express genes that allow the parasite to disseminate into the tissues and invade cells, we reasoned that genes preferentially expressed in trypomastigotes represent potential targets for immunization. Here we screened an epimastigote-subtracted trypomastigote cDNA expression library by genetic immunization, in order to find new vaccine candidates for Chagas´ disease. After two rounds of immunization and challenge with trypomastigotes, this approach led to the identification of a pool of 28 gene fragments that improved in vivo protection. Sequence analysis of these putative candidates revealed that 19 out of 28 (67.85%) of the genes were hypothetical proteins or unannotated T. cruzi open reading frames, which certainly would not have been identified by other methods of vaccine discovery.
Palabras clave: Trypanosma cruzi , Vaccine , Tripomastigote antigens , Expression Library Immunization
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/113605
URL: https://www.sciencedirect.com/science/article/pii/S0264410X0900005X
DOI: http://dx.doi.org/10.1016/j.vaccine.2008.12.056
Colecciones
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Alba Soto, Catalina Dirney; Tekiel, Valeria Sonia; González, Stella Maris; Postan, Miriam; Sanchez, Daniel Oscar; Identification of novel vaccine candidates for Chagas’ disease by immunization with sequential fractions of a trypomastigote cDNA expression library; Elsevier; Vaccine; 27; 9; 2-2009; 1323-1332
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