Artículo
An azoospermic man with a double-strand DNA break-processing deficiency in the spermatocyte nuclei: Case report
Sciurano, Roberta Beatriz
; Rahn, Irene Monica
; Pigozzi, Maria Ines
; Brugo Olmedo, S.; Solari, Alberto Juan
Fecha de publicación:
05/2006
Editorial:
Oxford University Press
Revista:
Human Reproduction
ISSN:
0268-1161
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
BACKGROUND: The mechanisms of meiotic arrest in human spermatogenesis are poorly known. METHODS AND RESULTS: A testicular biopsy from an azoospermic male showed complete spermatogenesis arrest at the spermatocyte stage, asynapsis, lack of formation of the XY body, partial reversion to a mitotic-like division and cell degeneration both at the prophase and at the abnormal cell divisions. Synaptonemal complex analysis showed minor segments of synapsis and mainly single axes. Fluorescent immunolocalization of meiotic proteins showed normal SYCP3, scarcity of SYCP1, null MLH1 foci, about 10 patches of -H2AX, abnormal presence of BRCA1 among autosomal axes, absence of RAD51 in early and advanced spermatocytes and permanence of -H2AX labelling up to the abnormal spermatocyte divisions that are the most advanced stage reached. There are at least six dominions of evenly packed chromatin resembling that of the normal XY body, but no true XY body. CONCLUSIONS: The protein phenotype and the fine structure of the nuclei are compatible with a deficiency of the processing of double-strand DNA breaks in the zygotene-like spermatocytes, but the features of this defect do not agree with Spo11, Sycp1, Atm and Dmc1 null mutations, which give absence of XY body, synapsis disturbances and spermatocyte apoptosis in mice.
Palabras clave:
FERTILITY
,
MEIOTIC ARREST
,
HUMAN SPERMATOGENESIS
Archivos asociados
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Identificadores
Colecciones
Articulos(INBIOMED)
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Sciurano, Roberta Beatriz; Rahn, Irene Monica; Pigozzi, Maria Ines; Brugo Olmedo, S.; Solari, Alberto Juan; An azoospermic man with a double-strand DNA break-processing deficiency in the spermatocyte nuclei: Case report; Oxford University Press; Human Reproduction; 21; 5; 5-2006; 1194-1203
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