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dc.contributor.author
Sciurano, Roberta Beatriz
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dc.contributor.author
Pigozzi, Maria Ines
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dc.contributor.author
Benavente, Ricardo
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dc.date.available
2020-09-08T18:01:16Z
dc.date.issued
2019-02
dc.identifier.citation
Sciurano, Roberta Beatriz; Pigozzi, Maria Ines; Benavente, Ricardo; Disassembly of the synaptonemal complex in chicken oocytes analyzed by super-resolution microscopy; Springer; Chromosoma; 128; 2-2019; 443–451
dc.identifier.issn
0009-5915
dc.identifier.uri
http://hdl.handle.net/11336/113523
dc.description.abstract
The synaptonemal complex is an evolutionarily conserved, supramolecular structure that holds the homologous chromosomes together during the pachytene stage of the first meiotic prophase. Among vertebrates, synaptonemal complex dynamics has been analyzed in mouse spermatocytes following the assembly of its components from leptotene to pachytene stages. With few exceptions, a detailed study of the disassembly of SCs and the behavior of SC components at recombination sites at the onset of diplotene has not been accomplished. Here, we describe for the first time the progressive disassembly of the SC in chicken oocytes during the initial steps of desynapsis using immunolocalization of specific SC proteins and super-resolution microscopy. We found that transverse filament protein SYCP1 and central element component SYCE3 remain associated with the lateral elements at the beginning of chromosomal axis separation. As the separation between lateral elements widens, these proteins eventually disappear, without any evidence of subsequent association. Our observations support the idea that post-translational modifications of the central region components have a role at the initial phases of the SC disassembly. At the crossover sites, signaled by persistent MLH1 foci, the central region proteins are no longer detected when the SYCP3-positive lateral elements are widely separated. These findings are indicative that SC disassembly follows a general pattern along the desynaptic bivalents. The present work shows that the use of avian oocytes at prophase I provides a valuable model to explore the time course and chromosomal localization of SC proteins and its relationship with local changes along meiotic bivalents.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MEIOSIS
dc.subject
SYNAPTONEMAL COMPLEX
dc.subject
CHICKEN
dc.subject
SUPER RESOLUTION MICROSCOPY
dc.subject.classification
Biología Reproductiva
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Disassembly of the synaptonemal complex in chicken oocytes analyzed by super-resolution microscopy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-03T16:57:00Z
dc.journal.volume
128
dc.journal.pagination
443–451
dc.journal.pais
Alemania
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dc.journal.ciudad
Berlín
dc.description.fil
Fil: Sciurano, Roberta Beatriz. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Cátedra de Histología, Citología y Embriología II; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil
Fil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: Benavente, Ricardo. Universität Würzburg; Alemania
dc.journal.title
Chromosoma
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00412-019-00693-w
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00412-019-00693-w
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