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dc.contributor.author
Itoh, Yuichiro
dc.contributor.author
Kampf, Kathy
dc.contributor.author
Pigozzi, Maria Ines
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dc.contributor.author
Arnold, Arthur P.
dc.date.available
2020-09-07T21:37:32Z
dc.date.issued
2009-05
dc.identifier.citation
Itoh, Yuichiro; Kampf, Kathy; Pigozzi, Maria Ines; Arnold, Arthur P.; Molecular cloning and characterization of the germline-restricted chromosome sequence in the zebra finch; Springer Verlag Berlín; Chromosoma; 118; 4; 5-2009; 527-536
dc.identifier.issn
0009-5915
dc.identifier.uri
http://hdl.handle.net/11336/113434
dc.description.abstract
The zebra finch (Taeniopygia guttata) germline-restricted chromosome (GRC) is the largest chromosome and has a unique system of transmission in germ cells. In the male, the GRC exists as a single heterochromatic chromosome in the germline and is eliminated from nuclei in late spermatogenesis. In the female, the GRC is bivalent and euchromatic and experiences recombination. These characteristics suggest a female-specific or female-beneficial function of the GRC. To shed light on the function of GRC, we cloned a portion of the GRC using random amplified polymorphic DNA-polymerase chain reaction and analyzed it using molecular genetic and cytogenetic methods. The GRC clone hybridized strongly to testis but not blood DNA in genomic Southern blots. In fluorescent in situ hybridization analysis on meiotic chromosomes from synaptonemal complex spreads, the probe showed hybridization across a large area of the GRC, suggesting that it contains repetitive sequences. We isolated a sequence homologous to the GRC from zebra finch chromosome 3 and a region of chicken chromosome 1 that is homologous to zebra finch chromosome 3; the phylogenetic analysis of these three sequences suggested that the GRC sequence and the zebra finch chromosome 3 sequence are most closely related. Thus, the GRC sequences likely originated from autosomal DNA and have evolved after the galliform-passeriform split. The present study provides a foundation for further study of the intriguing GRC.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Verlag Berlín
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
MEIOSIS
dc.subject
CHROMOSOME STRUCTURE
dc.subject
ZEBRA FINCH
dc.subject
AVES
dc.subject.classification
Genética y Herencia
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Molecular cloning and characterization of the germline-restricted chromosome sequence in the zebra finch
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-11T15:49:47Z
dc.identifier.eissn
1432-0886
dc.journal.volume
118
dc.journal.number
4
dc.journal.pagination
527-536
dc.journal.pais
Alemania
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dc.description.fil
Fil: Itoh, Yuichiro. University of California at Los Angeles; Estados Unidos
dc.description.fil
Fil: Kampf, Kathy. University of California at Los Angeles; Estados Unidos
dc.description.fil
Fil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: Arnold, Arthur P.. University of California at Los Angeles; Estados Unidos
dc.journal.title
Chromosoma
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00412-009-0216-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701497/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00412-009-0216-6
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