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dc.contributor.author
Slodowicz, Mariel Pamela
dc.contributor.author
Ceriani Nakamurakare, Esteban Daniel
dc.contributor.author
Carmaran, Cecilia Cristina
dc.contributor.author
Gonzalez Audino, Paola Andrea
dc.date.available
2020-09-04T18:14:18Z
dc.date.issued
2019-03
dc.identifier.citation
Slodowicz, Mariel Pamela; Ceriani Nakamurakare, Esteban Daniel; Carmaran, Cecilia Cristina; Gonzalez Audino, Paola Andrea; Sex pheromone component produced by microbial associates of the forest pest Megaplatypus mutatus; Wiley Blackwell Publishing, Inc; Entomologia Experimentalis et Applicata; 167; 3; 3-2019; 231-240
dc.identifier.issn
0013-8703
dc.identifier.uri
http://hdl.handle.net/11336/113282
dc.description.abstract
Megaplatypus mutatus (Chapuis) (Coleoptera: Platypodidae) is an ambrosia beetle native to South America that causes economic loss and was recently introduced to Italy, where it attacks and damages live poplar trees. Sulcatol and sulcatone are male-produced pheromone components involved in the mating process of M. mutatus. Their relative proportions are highly variable among insects, although the temporal pattern shows that initially only sulcatol is present, and sulcatone increases with time, until they are finally both depleted. Sulcatol and sulcatone may be produced de novo by the beetles, they may be produced by fungi, or both pathways may contribute to their production. Sulcatol is stored in the males’ hindgut but sulcatone is only present in emissions, so there is an oxidation process to transform the alcohol to the ketone before or during pheromone release. It is our hypothesis that fungi associated with M. mutatus are responsible for this process. In this work, we studied a possible contribution of associated microorganisms in the conversion of sulcatol into sulcatone and its consequent role in the temporal release pattern of these sex pheromone components observed in male insects. Moreover, we inhibited the postulated enzymes involved in this pheromone conversion process – 3-hydroxy-3-methyl-glutatyl-CoA reductase (HMGR) and P450 enzymes of a fungal strain – and added an antibiotic and a fungicide to the homogenate during sulcatol-sulcatone conversion. Among the fungal species, particular interest was given to Graphium basitruncatum (Matsush.) Seifert & Okada (Microascales), as it is present in male but not in female exoskeletons and in insect gallery samples, suggesting a possible different role in pherome production, as the male is the pheromone-producing sex. Several isolated strains were able to convert sulcatol to sulcatone, whereas the fungus G. basitruncatum showed the highest production of this ketone. Additionally, inhibition of P450 enzymes and HMGR from G. basitruncatum on this alcohol-ketone conversion demonstrated that HMGR is involved in sulcatone generation using sulcatol as precursor, and that P450 enzymes are not. Finally, sulcatone production diminished significantly in homogenized tissues of male and female M. mutatus following addition of an antibiotic and a fungicide. The results suggest that fungi associated with M. mutatus are involved in pheromone production.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AMBROSIA BEETLE
dc.subject
COLEOPTERA
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GRAPHIUM BASITRUNCATUM
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PLATYPODIDAE
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POPLAR TREE
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SULCATOL
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SULCATONE
dc.subject.classification
Micología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Sex pheromone component produced by microbial associates of the forest pest Megaplatypus mutatus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-08T18:55:56Z
dc.journal.volume
167
dc.journal.number
3
dc.journal.pagination
231-240
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Slodowicz, Mariel Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
dc.description.fil
Fil: Ceriani Nakamurakare, Esteban Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria; Argentina
dc.description.fil
Fil: Carmaran, Cecilia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria; Argentina
dc.description.fil
Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
dc.journal.title
Entomologia Experimentalis et Applicata
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/eea.12744
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1111/eea.12744
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