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dc.contributor.author
Martinez, Sol Romina
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dc.contributor.author
Durantini, Andres Matías
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dc.contributor.author
Becerra, María Cecilia
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dc.contributor.author
Cosa, Gonzalo
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dc.date.available
2020-08-31T21:02:49Z
dc.date.issued
2020-07
dc.identifier.citation
Martinez, Sol Romina; Durantini, Andres Matías; Becerra, María Cecilia; Cosa, Gonzalo; Real-time single-cell imaging reveals accelerating lipid peroxyl radical formation in Escherichia coli triggered by a fluoroquinolone antibiotic; American Chemical Society; ACS Infectious Diseases; 7-2020; 1-11
dc.identifier.issn
2373-8227
dc.identifier.uri
http://hdl.handle.net/11336/112847
dc.description.abstract
Formation of reactive oxygen species (ROS) induced by bactericidal antibiotics has been associated with a common, non-specific, mechanism of cellular death. Herein we report real-time single cell fluorescence studies on E. coli stained with a fluorogenic probe for lipid peroxyl radicals showing generation of this form of ROS when exposed to the minimum inhibitory concentration (MIC) and 10xMIC of the fluoroquinolone antibiotic ciprofloxacin (3 and 30 μM, respectively). Single-cell intensity-time trajectories show an induction period followed by an accelerating phase for cells treated with antibiotic, where initial and maximum intensity achieved following 3.5 h incubation with antibiotic showed dose dependent average values. A large fraction of bacteria remains viable after the studies, indicating ROS formation is occurring a priori of cell death. Punctate structures are observed, consistent with membrane blebbing. Addition of a membrane embedding lipid peroxyl radical scavenger, an α-tocopherol analogue, to the media increased the MIC of ciprofloxacin. Lipid peroxyl radical formation precedes E. coli cell death and may be invoked in a cascade event including membrane disruption and consequent cell wall permeabilization. Altogether, our work illustrates that lipid peroxidation is caused by ciprofloxacin in E. coli, and suppressed by α-tocopherol analogues. Lipid peroxidation may be invoked in a cascade event including membrane disruption and consequent cell wall permeabilization. Our work provides a methodology to assess antibiotic induced membrane peroxidation at the single cell level, this methodology provides opportunities to explore the scope and nature of lipid peroxidation in antibiotic induced cell lethality.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
REACTIVE OXYGEN SPECIES
dc.subject
LIPID PEROXIDATION
dc.subject
FLUOROGENIC SENSOR
dc.subject
CELL VIABILITY
dc.subject.classification
Otras Ciencias Químicas
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Real-time single-cell imaging reveals accelerating lipid peroxyl radical formation in Escherichia coli triggered by a fluoroquinolone antibiotic
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-08-24T18:08:15Z
dc.journal.pagination
1-11
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Martinez, Sol Romina. McGill University; Canadá. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
dc.description.fil
Fil: Durantini, Andres Matías. McGill University; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil
Fil: Cosa, Gonzalo. McGill University; Canadá
dc.journal.title
ACS Infectious Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsinfecdis.0c00317
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acsinfecdis.0c00317
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