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dc.contributor.author
Sosa Alderete, Lucas Gastón
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dc.contributor.author
Racagni, Graciela Esther
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dc.contributor.author
Agostini, Elizabeth
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dc.contributor.author
Medina, María I.
dc.date.available
2020-08-31T14:08:58Z
dc.date.issued
2019-10
dc.identifier.citation
Sosa Alderete, Lucas Gastón; Racagni, Graciela Esther; Agostini, Elizabeth; Medina, María I.; Is the PLC pathway involved in the response to phenol treatment in tobacco hairy roots?; Springer; In Vitro Cellular; 55; 5; 10-2019; 549-557
dc.identifier.issn
1054-5476
dc.identifier.uri
http://hdl.handle.net/11336/112711
dc.description.abstract
Phospholipids and phospholipases play important roles in several cellular processes and responses to adverse growth conditions. However, the mechanism of action of phosphatidylinositol-specific phospholipase C (PI-PLC), and its resulting minor lipid function in response to pollutant-induced stress, remains to be elucidated. In this work, we studied the effects of phenol treatment on the PLC pathway, using two lines of hairy root cultures (HRs) from Nicotiana tabacum as plant model systems: wild-type (WT) and double-transgenic (DT) HRs. We quantified several product formations such as PIP, PIP 2 , DGPP, and PA, which are mainly synthesized by specific lipid kinases belonging to the PLC pathway. In both HRs, phenol treatment significantly increased the formation of these compounds in a differential manner. In WT HRs, PA formation was twofold higher than in control. PIP 2 and PIP levels were about onefold higher than those of the controls while DGPP levels increased by 50%. In DT HRs, PIP levels were onefold higher than those of the controls while PIP 2 , DGPP, and PA levels increased by 120%. Phenol treatment also upregulated the PLC4 gene expression mainly in the first hours of exposure in both HRs, while the DGK1 gene expression was only upregulated in WT HRs after 24 h of treatment. These results show an active participation of the PLC pathway under phenol treatment suggesting that this signal pathway could be important in plant cell responses to phenol-induced stress.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
LIPID KINASES
dc.subject
PHENOL TREATMENT
dc.subject
PLC PATHWAY
dc.subject
TOBACCO HAIRY ROOTS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Is the PLC pathway involved in the response to phenol treatment in tobacco hairy roots?
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-08-20T20:25:57Z
dc.identifier.eissn
1475-2689
dc.journal.volume
55
dc.journal.number
5
dc.journal.pagination
549-557
dc.journal.pais
Alemania
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dc.description.fil
Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
dc.description.fil
Fil: Racagni, Graciela Esther. Universidad Nacional de Río Cuarto; Argentina
dc.description.fil
Fil: Agostini, Elizabeth. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
dc.description.fil
Fil: Medina, María I.. Universidad Nacional de Río Cuarto; Argentina
dc.journal.title
In Vitro Cellular
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11627-019-09968-4
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11627-019-09968-4
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