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dc.contributor.author
Lanfranconi, Mariana Patricia
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dc.contributor.author
Alvarez, Adrián F.
dc.contributor.author
Alvarez, Hector Manuel
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dc.date.available
2020-08-28T19:37:19Z
dc.date.issued
2015-12
dc.identifier.citation
Lanfranconi, Mariana Patricia; Alvarez, Adrián F.; Alvarez, Hector Manuel; Identification of genes coding for putative wax ester synthase/diacylglycerol acyltransferase enzymes in terrestrial and marine environments; Springer Verlag Berlín; AMB Express; 5; 42; 12-2015; 1-13
dc.identifier.issn
2191-0855
dc.identifier.uri
http://hdl.handle.net/11336/112688
dc.description.abstract
Synthesis of neutral lipids such as triacylglycerols (TAG) and wax esters (WE) is catalyzed in bacteria by wax ester synthase/diacylglycerol acyltransferase enzymes (WS/DGAT). We investigated the diversity of genes encoding this enzyme in contrasting natural environments from Patagonia (Argentina). The content of petroleum hydrocarbons in samples collected from oil-producing areas was measured. PCR-based analysis covered WS/DGAT occurrence in marine sediments and soil. No product was obtained in seawater samples. All clones retrieved from marine sediments affiliated with gammaproteobacterial sequences and within them, most phylotypes formed a unique cluster related to putative WS/DGAT belonging to marine OM60 clade. In contrast, soils samples contained phylotypes only related to actinomycetes. Among them, phylotypes affiliated with representatives largely or recently reported as oleaginous bacteria, as well as with others considered as possible lipid-accumulating bacteria based on the analysis of their annotated genomes. Our study shows for the first time that the environment could contain a higher variety of ws/ dgat than that reported from bacterial isolates. The results of this study highlight the relevance of the environment in a natural process such as the synthesis and accumulation of neutral lipids. Particularly, both marine sediments and soil may serve as a useful source for novel WS/DGAT with biotechnological interest.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Verlag Berlín
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
COASTAL MARINE SEDIMENTS
dc.subject
ENVIRONMENTAL DIVERSITY
dc.subject
NEUTRAL LIPIDS
dc.subject
POLLUTED ARID SOILS
dc.subject
WS/DGAT ENZYMES
dc.subject.classification
Biotecnología Medioambiental
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dc.subject.classification
Biotecnología del Medio Ambiente
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Identification of genes coding for putative wax ester synthase/diacylglycerol acyltransferase enzymes in terrestrial and marine environments
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-08-28T18:40:36Z
dc.journal.volume
5
dc.journal.number
42
dc.journal.pagination
1-13
dc.journal.pais
Suiza
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dc.journal.ciudad
Zug
dc.description.fil
Fil: Lanfranconi, Mariana Patricia. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Alvarez, Adrián F.. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
AMB Express
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13568-015-0128-1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://amb-express.springeropen.com/articles/10.1186/s13568-015-0128-1
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