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dc.contributor.author
Hampel, Sabrina
dc.contributor.author
Steitz, Jan Patrick
dc.contributor.author
Baierl, Anna
dc.contributor.author
Lehwald, Patrizia
dc.contributor.author
Wiesli, Luzia
dc.contributor.author
Richter, Michael
dc.contributor.author
Fries, Alexander Erich
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dc.contributor.author
Pohl, Martina
dc.contributor.author
Schneider, Gunter
dc.contributor.author
Dobritzsch, Doreen
dc.contributor.author
Müller, Michael
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dc.date.available
2020-08-27T14:24:46Z
dc.date.issued
2018-11
dc.identifier.citation
Hampel, Sabrina; Steitz, Jan Patrick; Baierl, Anna; Lehwald, Patrizia; Wiesli, Luzia; et al.; Structural and Mutagenesis Studies of the Thiamine-Dependent, Ketone-Accepting YerE from Pseudomonas protegens; Wiley VCH Verlag; Chembiochem; 19; 21; 11-2018; 2283-2292
dc.identifier.issn
1439-4227
dc.identifier.uri
http://hdl.handle.net/11336/112531
dc.description.abstract
A wide range of thiamine diphosphate (ThDP)‐dependent enzymes catalyze the benzoin‐type carboligation of pyruvate with aldehydes. A few ThDP‐dependent enzymes, such as YerE from Yersinia pseudotuberculosis (YpYerE), are known to accept ketones as acceptor substrates. Catalysis by YpYerE gives access to chiral tertiary alcohols, a group of products difficult to obtain in an enantioenriched form by other means. Hence, knowledge of the three‐dimensional structure of the enzyme is crucial to identify structure–activity relationships. However, YpYerE has yet to be crystallized, despite several attempts. Herein, we show that a homologue of YpYerE, namely, PpYerE from Pseudomonas protegens (59 % amino acid identity), displays similar catalytic activity: benzaldehyde and its derivatives as well as ketones are converted into chiral 2‐hydroxy ketones by using pyruvate as a donor. To enable comparison of aldehyde‐ and ketone‐accepting enzymes and to guide site‐directed mutagenesis studies, PpYerE was crystallized and its structure was determined to a resolution of 1.55 Å.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ASYMMETRIC SYNTHESIS
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BIOCATALYSIS
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BIOSYNTHESIS
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C−C COUPLING
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TERTIARY ALCOHOLS
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Structural and Mutagenesis Studies of the Thiamine-Dependent, Ketone-Accepting YerE from Pseudomonas protegens
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-04T20:51:31Z
dc.journal.volume
19
dc.journal.number
21
dc.journal.pagination
2283-2292
dc.journal.pais
Alemania
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dc.journal.ciudad
Weinheim
dc.description.fil
Fil: Hampel, Sabrina. Albert Ludwigs University of Freiburg; Alemania
dc.description.fil
Fil: Steitz, Jan Patrick. Albert Ludwigs University of Freiburg; Alemania
dc.description.fil
Fil: Baierl, Anna. IBG-1: Biotechnology. Forschungszentrum Jülich GmbH Wilhelm-Johnen Straße; Alemania
dc.description.fil
Fil: Lehwald, Patrizia. Albert Ludwigs University of Freiburg; Alemania
dc.description.fil
Fil: Wiesli, Luzia. Swiss Federal Laboratories For Materials Science And Technology; Suiza
dc.description.fil
Fil: Richter, Michael. Swiss Federal Laboratories For Materials Science And Technology; Suiza. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Branch BioCa; Alemania
dc.description.fil
Fil: Fries, Alexander Erich. Albert Ludwigs University of Freiburg; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Pohl, Martina. IBG-1: Biotechnology. Forschungszentrum Jülich GmbH Wilhelm-Johnen Straße; Alemania
dc.description.fil
Fil: Schneider, Gunter. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
dc.description.fil
Fil: Dobritzsch, Doreen. Uppsala Universitet; Suecia
dc.description.fil
Fil: Müller, Michael. Albert Ludwigs University of Freiburg; Alemania
dc.journal.title
Chembiochem
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cbic.201800325
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cbic.201800325
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