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Artículo

Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis

Eirin, Maria EmiliaIcon ; Macías, Analia Florencia; Magnano, Gabriel Gustavo; Morsella, Claudia; Mendez, Laura; Blanco, Federico CarlosIcon ; Bianco, María Verónica; Severina, Walter; Alito, Alicia; Pando, María de los ÁngelesIcon ; Singh, Mahavir; Spallek, Ralph; Paolicchi, Fernando Alberto; Bigi, FabianaIcon ; Cataldi, Ángel AdriánIcon
Fecha de publicación: 06/2015
Editorial: Churchill Livingstone
Revista: Tuberculosis (Edinb)
ISSN: 1472-9792
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnología Agropecuaria

Resumen

Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis),responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cellmediated immunity under the exposure to protein purified derivative tuberculin (PPD), a complex andpoorly characterized reagent. The cross-reactivity to non-tuberculous mycobacterium species (falsepositiveresults) has been crucial to develop a more proper antigen. In the present study, we selected sixM. bovis Open Reading Frames (Mb1992, Mb2031c, Mb2319, Mb2843c, Mb2845c and Mb3212c) by insilicoanalysis and evaluated them in experimental and natural infection; none of these antigens hadbeen previously assessed as diagnostic antigens for bTB. The reactivity performance was tested in animalswith both positive and negative Tuberculin Skin Test (TST) results as well as in cattle infected withMycobacterium avium subesp. paratuberculosis (MAP). The six recombinant antigens individually inducedan IFN-g response, with overall responder frequency ranging from 18.3 to 31%. Mb2845c was the mostvaluable antigen with the potential to discriminate TST-positive cattle from either TST-negative or MAPinfected animals. Mb2845c showed similar performance to that observed with ESAT-6 and PPD-B amongTST and MTC specific-PCR positive animals, although this result needs to be proven in further studieswith a higher sample size. Our data confirm the feacibility to implement bioinformatic screening toolsand suggest Mb2845c as a potential diagnostic antigen to be tested in protein cocktails to evaluate theircontribution to bTB diagnosis.
Palabras clave: TUBERCULOSIS , BOVINA , DIAGNÓSTICO , INTERFERON
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/112130
URL: http://www.sciencedirect.com/science/article/pii/S1472979215207688
DOI: http://dx.doi.org/10.1016/j.tube.2015.07.009
Colecciones
Articulos(INBIRS)
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS EN RETROVIRUS Y SIDA
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Eirin, Maria Emilia; Macías, Analia Florencia; Magnano, Gabriel Gustavo; Morsella, Claudia; Mendez, Laura; et al.; Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis; Churchill Livingstone; Tuberculosis (Edinb); 95; 6; 6-2015; 795-801
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