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dc.contributor.author
Recouvreux, Maria Victoria
dc.contributor.author
Guida, María Clara
dc.contributor.author
Rifkin, Daniel B.
dc.contributor.author
Becu, Damasia
dc.contributor.author
Diaz, Graciela Susana
dc.date.available
2017-01-12T17:59:07Z
dc.date.issued
2011-07
dc.identifier.citation
Recouvreux, Maria Victoria; Guida, María Clara; Rifkin, Daniel B.; Becu, Damasia; Diaz, Graciela Susana; Active and Total Transforming Growth Factor-{beta}1 are Differentially Regulated by Dopamine and Estradiol in the Pituitary; Endocrine Society; Endocrinology; 152; 7; 7-2011; 2722-2730
dc.identifier.issn
0013-7227
dc.identifier.uri
http://hdl.handle.net/11336/11210
dc.description.abstract
Dopamine, acting through the dopamine type 2 receptor (Drd2), is the main inhibitor of pituitary prolactin (PRL) secretion and lactotroph proliferation. TGF-β1 is involved, at least in part, in mediating these actions. It was described that TGF-β1 synthesis in rat pituitary lactotrophs is up-regulated by dopamine and down-regulated by estradiol. TGF-β1 is secreted as a large latent complex. The local regulation of cytokine activation in the pituitary has not yet been explored. In this work, we studied pituitary active and total TGF-β1 content, as well as TGF-β1 mRNA, and the in vivo role of dopamine and estradiol on pituitary TGF-β1 levels. Adult female mice (wild type), and female mice with a null mutation in the Drd2 (Drd2−/−), were used. The loss of dopaminergic tone induced a decrease in TGF-β1 mRNA expression, in active and total cytokine content, and in TGF-β type II receptor expression. Dopamine regulation of pituitary TGF-β1 activation process was inferred by the inhibition of active cytokine by in vivo sulpiride treatment. Interestingly, in the absence of dopaminergic tone, estradiol induced a strong increase in active TGF-β1. PRL secretion correlated with active, but not total cytokine. TGF-β1 inhibitory action on lactotroph proliferation and PRL secretion was decreased in Drd2−/− pituitary cells, in correlation with decreased TGF-β type II receptor. The study of the TGF-β1 activation process and its regulation is essential to understand the cytokine activity. As an intermediary of dopamine inhibition of lactotroph function, TGF-β1 and local activators may be important targets in the treatment of dopamine agonist-resistant prolactinomas.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Active Tgfb1
dc.subject
Pituitary
dc.subject
Estradiol
dc.subject
Dopamine
dc.subject.classification
Fisiología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Active and Total Transforming Growth Factor-{beta}1 are Differentially Regulated by Dopamine and Estradiol in the Pituitary
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-01-11T20:31:46Z
dc.identifier.eissn
1945-7170
dc.journal.volume
152
dc.journal.number
7
dc.journal.pagination
2722-2730
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Recouvreux, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Guida, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Rifkin, Daniel B.. University Of New York; Estados Unidos
dc.description.fil
Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://press.endocrine.org/doi/10.1210/en.2010-1464
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1210/en.2010-1464
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2010-1464


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