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dc.contributor.author
Gutiérrez, Alicia Beatriz
dc.contributor.author
Sambuco, Lorena Andrea
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Alvarez, Laura
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Núñez, Mariel Alejandra
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Bergoc, Rosa Maria
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Zotta, Elsa
dc.contributor.author
Martín, Gabriela
dc.contributor.author
Randi, Andrea Silvana
dc.date.available
2020-08-03T17:35:22Z
dc.date.issued
2020-01
dc.identifier.citation
Gutiérrez, Alicia Beatriz; Sambuco, Lorena Andrea; Alvarez, Laura; Núñez, Mariel Alejandra; Bergoc, Rosa Maria; et al.; Expression of estrogen receptor α variants and c-Fos in rat mammary gland and tumors; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 199; 1-2020; 1-12
dc.identifier.issn
0960-0760
dc.identifier.uri
http://hdl.handle.net/11336/110791
dc.description.abstract
Breast cancer is currently the leading cause of cancer death among women worldwide. AP-1 (c-Fos/c-Jun) isassociated with proliferation and survival, while cytoplasmic c-Fos activates phospholipid synthesis in cellsinduced to differentiate or grow. Estrogen receptor α 46 (ERα46) is a splice variant of full-length ERα66 and it isknown that it has an inhibitory role in cancer cell growth. We investigated c-Fos localization, its relationship toAP-1, the non genomic pathway of phospho-Tyr537-ERα66, as well as ERα46 and ERα66 isoforms in ratmammary gland development and carcinogenic transformation, and in mammary tumors. Female rats wereinjected: a) saline solution (Control mammary gland, CMG) or b) N-Nitroso-N-methyl urea (NMU), and sampleswere taken at 60, 90, 120 and 150 days of life. In addition, we analyzed hormone-dependent (HD) and in-dependent (HI) tumors in ovariectomized rats, and intact tumors (IT) in non-ovariectomized ones. Our resultsshow that, in CMG, nuclear c-Fos and proliferation decreased with age, AP-1 content was low, and nuclearERα46/ERα66 ratio was higher than 1. In NMU, nuclear c-Fos and proliferation increased with carcinogenictransformation, AP-1 content was high, and nuclear ERα46/ERα66 was below 1. As tumor grade increased,proliferation, nuclear c-Fos and AP-1 expression were negatively associated to nuclear ERα46/ERα66 in IT. InHD, nuclear ERα46/ERα66, nuclear c-Fos expression, AP-1 levels and proliferation were lower than in HI, whosegrowth is estrogen-independent. Phospho-Tyr537-ERα66 content and ERK1/2 activation were associated withAP-1 levels and cell proliferation. Collectively, our findings support the notion that variant detection andERα46/ERα66 ratio could shed light on the role of ERα isoforms in mammary gland transformation and thebehavior of ERα positive mammary tumors.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ESTROGEN RECEPTOR ALPHA VARIANTS
dc.subject
c-FOS
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MAMMARY TUMORS
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MAMMARY GLAND
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AP-1
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CELL PROLIFERATION
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PCNA
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ER ALPHA 66
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ER ALPHA 46
dc.subject.classification
Otras Ciencias de la Salud
dc.subject.classification
Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Expression of estrogen receptor α variants and c-Fos in rat mammary gland and tumors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-01T17:03:43Z
dc.journal.volume
199
dc.journal.pagination
1-12
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gutiérrez, Alicia Beatriz. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Sambuco, Lorena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Físico Matemática; Argentina
dc.description.fil
Fil: Alvarez, Laura. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana. Cátedra de Química Biologica; Argentina
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Fil: Núñez, Mariel Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Físico Matemática; Argentina
dc.description.fil
Fil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Físico Matemática; Argentina
dc.description.fil
Fil: Zotta, Elsa. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
dc.description.fil
Fil: Martín, Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Físico Matemática; Argentina
dc.description.fil
Fil: Randi, Andrea Silvana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal of Steroid Biochemistry and Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jsbmb.2020.105594
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0960076019304935
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