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dc.contributor.author
Marinangeli, Pablo Alejandro
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dc.contributor.other
Lambardi, Maurizio
dc.contributor.other
Ozudogru, Elif Aylin
dc.contributor.other
Jain, S. Mohan
dc.date.available
2020-07-22T18:22:21Z
dc.date.issued
2013
dc.identifier.citation
Marinangeli, Pablo Alejandro; Micropropagation of Onion (Allium cepa L.) from Immature Inflorescences; Humana Press; 2013; 319-328
dc.identifier.isbn
978-1-62703-073-1
dc.identifier.uri
http://hdl.handle.net/11336/109945
dc.description.abstract
In vitro plant production by direct organogenesis from immature flower heads is an ideal approach for clonal propagation of onions (Allium cepa L.). This technique ensures genetic stability, high propagation rate, and maintains donor plant of explants with an advantage over other means of in vitro regeneration. Onion micropropagation is usually applied in breeding programs, maintenance, and multiplication of cytoplasmic-male sterile lines for hybrid production, germplasm conservation, and as a tool for the application of other biotechniques. For in vitro culture, mature onion bulbs are induced to reproductive phase by vernalization and forced to inflorescence initiation. Immature umbels are dissected from bulbs or cut directly when they appear from the pseudostem among the leaves. Disinfected inflorescences are cultivated in BDS basal medium supplemented with 30 g/L sucrose, 0.1 mg L naphtalene acetic acid, 1 mg/L N6 benzyladenine, and 8 g/L agar, pH 5.5, under 16 h photoperiod white fluorescent light (PPD : 50-70 umol /m2s) for 35 days. The regenerated shoot clumps are divided and subculture under the same condition. For bulbification phase, the individual shoots are cultured in BDS basal medium containing 90 g/L sucrose, without plant growth regulators, pH 5.5, under 16 h photoperiod. Microbulbs can be directly cultivated ex vitro without acclimation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
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dc.relation
https://link.springer.com/bookseries/7651
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Micropropagation
dc.subject
In vitro bulbification
dc.subject
Onion Allium cepa
dc.subject
Immature inflorescence
dc.subject.classification
Biotecnología Agrícola y Biotecnología Alimentaria
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dc.subject.classification
Biotecnología Agropecuaria
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Micropropagation of Onion (Allium cepa L.) from Immature Inflorescences
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/bookPart
dc.type
info:ar-repo/semantics/parte de libro
dc.date.updated
2020-06-22T14:15:12Z
dc.journal.pagination
319-328
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-1-62703-074-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007/978-1-62703-074-8_25
dc.conicet.paginas
490
dc.source.titulo
Protocols for Micropropagation of Selected Economically-Important Horticultural Plant
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