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dc.contributor.author
Quintela, Oscar
dc.contributor.author
Lendoiro, Elena
dc.contributor.author
Cruz, Angelines
dc.contributor.author
de Castro, Ana
dc.contributor.author
Quevedo, Mario Alfredo
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dc.contributor.author
Jurado, Carmen
dc.contributor.author
López-Rivadulla, Manuel
dc.date.available
2020-07-21T21:44:58Z
dc.date.issued
2010-01
dc.identifier.citation
Quintela, Oscar; Lendoiro, Elena; Cruz, Angelines; de Castro, Ana; Quevedo, Mario Alfredo; et al.; Hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC-MS/MS) determination of cocaine and its metabolites benzoylecgonine, ecgonine methyl ester, and cocaethylene in hair samples; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 396; 5; 1-2010; 1703-1712
dc.identifier.issn
1618-2642
dc.identifier.uri
http://hdl.handle.net/11336/109828
dc.description.abstract
This study reports the development and validation of a method using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/ MS) for the analysis of cocaine and its metabolites benzoylecgonine (BE), ecgonine methyl ester (EME), and cocaethylene (CE) in hair samples. Decontamination was performed as follows: Firstly, the aliquot of hair was briefly rinsed with 2 mL dichloromethane, then was washed three times with 10 mL 0.01 M phosphate buffer, pH 6, for 15 min, followed by 2 mL 2-propanol for less than 2 min, and, finally, a last rinse with 2 mL dichloromethane was again done. Cocaine compounds were extracted from 10 mg of hair by incubation with 2 mL 0.1 M HCl at 50 °C for 12 h and purified by solid phase extraction with Oasis MCX cartridges. Analysis was performed by LC-MS/ MS using an Atlantis HILIC silica chromatographic column. The method was fully validated. Linearity was established over the concentration range 0.020?10.0 ng/mg for cocaine (COC), 0.010?10.0 ng/mg for BE and CE, and 0.005?2.0 ng/mg for EME, and the correlation coefficients were all >0.99. Extraction efficiency was >70% for all analytes. Limits of detection were 0.0005 ng/mg for CE and 0.001 ng/mg for the other analytes (COC, BE, and EME). Lower limits of quantification were the lowest points of the calibration curves with acceptable accuracy and precision (coefficient of variation ≤20%). Intra- and inter-day imprecision ranged between 1.5% and 9.5% and 0.7% and 12.6%, respectively. Intra- and inter-day inaccuracy ranged from 0.5% to 12.3% and from 0.7% to 7.1%, respectively. With regard to matrix effects, suppression was <27.5% in all cases. The method was applied to the analysis of several samples derived from forensic cases.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Heidelberg
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hydrophilic interaction
dc.subject
Cocaine and metabolites
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Ecgonine methyl ester
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Hair analysis
dc.subject.classification
Toxicología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC-MS/MS) determination of cocaine and its metabolites benzoylecgonine, ecgonine methyl ester, and cocaethylene in hair samples
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-20T20:22:02Z
dc.identifier.eissn
1618-2650
dc.journal.volume
396
dc.journal.number
5
dc.journal.pagination
1703-1712
dc.journal.pais
Alemania
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dc.journal.ciudad
Heidelberg
dc.description.fil
Fil: Quintela, Oscar. Universidad de Santiago de Compostela. Instituto de Medicina Legal; España
dc.description.fil
Fil: Lendoiro, Elena. Universidad de Santiago de Compostela. Instituto de Medicina Legal; España
dc.description.fil
Fil: Cruz, Angelines. Universidad de Santiago de Compostela. Instituto de Medicina Legal; España
dc.description.fil
Fil: de Castro, Ana. Universidad de Santiago de Compostela. Instituto de Medicina Legal; España
dc.description.fil
Fil: Quevedo, Mario Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
dc.description.fil
Fil: Jurado, Carmen. National Institute of Toxicology and Forensic Sciences; España
dc.description.fil
Fil: López-Rivadulla, Manuel. Universidad de Santiago de Compostela. Instituto de Medicina Legal; España
dc.journal.title
Analytical and Bioanalytical Chemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00216-009-3393-5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00216-009-3393-5
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