Artículo
Design of a new electrogenerated polyquinone film substituted with glutathione. Towards direct electrochemical biosensors
Reisberg, S.; Acevedo, Diego Fernando
; Korovitch, A.; Piro, B.; Noel, V.; Buchet, I.; Tran, L. D.; Barbero, César Alfredo
; Pham, M. C.
Fecha de publicación:
01/2010
Editorial:
Elsevier Science
Revista:
Talanta
ISSN:
0039-9140
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We developed a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity in neutral buffered solution, due to the embedded quinone group, and is able to covalently graft amino-modified DNA probe strands. It is shown that the bioelectrode presents positive current change following DNA hybridization. This makes a “signal-on” direct electrochemical DNA sensor. The results were obtained with low target concentration (50 nM) and the selectivity is excellent as a single-mismatch sequence can be discriminated from the full-complementary target
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Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Reisberg, S.; Acevedo, Diego Fernando; Korovitch, A.; Piro, B.; Noel, V.; et al.; Design of a new electrogenerated polyquinone film substituted with glutathione. Towards direct electrochemical biosensors; Elsevier Science; Talanta; 80; 3; 1-2010; 1318-1325
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